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Appl Environ Microbiol

Title:		Expression and secretion of Bacillus amyloliquefaciens alpha-amylase by using the yeast pheromone alpha-factor promoter and leader sequence in Saccharomyces cerevisiae
Author(s):		Southgate VJ; Steyn AJ; Pretorius IS; Van Vuuren HJ;
Address:		"Department of Microbiology, University of Stellenbosch, South Africa"
Journal Title:		Appl Environ Microbiol
Year:		1993
Volume:		59
Issue:		4
Page Number:		1253 - 1258
DOI:		10.1128/aem.59.4.1253-1258.1993
ISSN/ISBN:		0099-2240 (Print) 1098-5336 (Electronic) 0099-2240 (Linking)
Abstract:		"Replacement of the regulatory and secretory signals of the alpha-amylase gene (AMY) from Bacillus amylolique-faciens with the complete yeast pheromone alpha-factor prepro region (MF alpha 1p) resulted in increased levels of extracellular alpha-amylase production in Saccharomyces cerevisiae. However, the removal of the (Glu-Ala)2 peptide from the MF alpha 1 spacer region (Lys-Arg-Glu-Ala-Glu-Ala) yielded decreased levels of extracellular alpha-amylase"
Keywords:		"Bacillus/*enzymology/genetics Base Sequence Gene Expression Regulation, Enzymologic Mating Factor Molecular Sequence Data Peptides/*genetics Saccharomyces cerevisiae/*enzymology/genetics Transcription, Genetic Transfection alpha-Amylases/genetics/*metabol;"
Notes:		"MedlineSouthgate, V J Steyn, A J Pretorius, I S Van Vuuren, H J eng Research Support, Non-U.S. Gov't 1993/04/01 Appl Environ Microbiol. 1993 Apr; 59(4):1253-8. doi: 10.1128/aem.59.4.1253-1258.1993"