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Science

Title:		Mediation of the attachment or fusion step in vesicular transport by the GTP-binding Ypt1 protein
Author(s):		Segev N;
Address:		"Department of Pharmacological and Physiological Sciences, University of Chicago, IL 60637"
Journal Title:		Science
Year:		1991
Volume:		252
Issue:		5012
Page Number:		1553 - 1556
DOI:		10.1126/science.1904626
ISSN/ISBN:		0036-8075 (Print) 0036-8075 (Linking)
Abstract:		"The function of the guanosine triphosphate (GTP)-binding protein Ypt1 in regulating vesicular traffic was studied in a cell-free system that reconstitutes transport from the endoplasmic reticulum to the Golgi. Blocking the Ypt1 protein activity resulted in accumulation of vesicles that act as an intermediate passing between the two compartments. The Ypt1 protein was found on the outer side of these vesicles. The transport process is completed by fusion of these vesicles with the acceptor compartment, and Ypt1 protein activity was needed for this step. Thus, a specific GTP-binding protein is required for either attachment or fusion (or both) of secretory vesicles with the acceptor compartment during protein secretion"
Keywords:		Cell-Free System Endoplasmic Reticulum/metabolism Fungal Proteins/*metabolism GTP-Binding Proteins/*metabolism Glycosylation Golgi Apparatus/metabolism Kinetics Mating Factor Microsomes/metabolism Peptides/genetics Protein Precursors/metabolism Protein Pr;
Notes:		"MedlineSegev, N eng Research Support, Non-U.S. Gov't 1991/06/14 Science. 1991 Jun 14; 252(5012):1553-6. doi: 10.1126/science.1904626"