| Title: | Genome-wide location and function of DNA binding proteins |
| Author(s): | Ren B; Robert F; Wyrick JJ; Aparicio O; Jennings EG; Simon I; Zeitlinger J; Schreiber J; Hannett N; Kanin E; Volkert TL; Wilson CJ; Bell SP; Young RA; |
| Address: | "Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA" |
| DOI: | 10.1126/science.290.5500.2306 |
| ISSN/ISBN: | 0036-8075 (Print) 0036-8075 (Linking) |
| Abstract: | "Understanding how DNA binding proteins control global gene expression and chromosomal maintenance requires knowledge of the chromosomal locations at which these proteins function in vivo. We developed a microarray method that reveals the genome-wide location of DNA-bound proteins and used this method to monitor binding of gene-specific transcription activators in yeast. A combination of location and expression profiles was used to identify genes whose expression is directly controlled by Gal4 and Ste12 as cells respond to changes in carbon source and mating pheromone, respectively. The results identify pathways that are coordinately regulated by each of the two activators and reveal previously unknown functions for Gal4 and Ste12. Genome-wide location analysis will facilitate investigation of gene regulatory networks, gene function, and genome maintenance" |
| Keywords: | "Binding Sites Cell Cycle DNA, Fungal/genetics/metabolism DNA-Binding Proteins/*metabolism Fungal Proteins/*metabolism Galactose/metabolism *Gene Expression Profiling *Gene Expression Regulation, Fungal Genes, Fungal *Genome, Fungal Mating Factor Oligonucl;" |
| Notes: | "MedlineRen, B Robert, F Wyrick, J J Aparicio, O Jennings, E G Simon, I Zeitlinger, J Schreiber, J Hannett, N Kanin, E Volkert, T L Wilson, C J Bell, S P Young, R A eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 2000/12/23 Science. 2000 Dec 22; 290(5500):2306-9. doi: 10.1126/science.290.5500.2306" |
