| Title: | The early transcriptome response of cassava (Manihot esculenta Crantz) to mealybug (Phenacoccus manihoti) feeding |
| Author(s): | Rauwane ME; Odeny DA; Millar I; Rey C; Rees J; |
| Address: | "Biotechnology Platform, Agricultural Research Council, Onderstepoort, South Africa. University of the Witwatersrand, Johannesburg, South Africa. ICRISAT-Nairobi, Nairobi, Kenya. Biosystematics Division, Plant Protection Research Institute, Agricultural Research Council, Queenswood, Pretoria, South Africa" |
| DOI: | 10.1371/journal.pone.0202541 |
| ISSN/ISBN: | 1932-6203 (Electronic) 1932-6203 (Linking) |
| Abstract: | "The mealybug, Phenacoccus manihoti, is a leading pest of cassava (Manihot esculenta Crantz), damaging this crop globally. Although the biological control of this mealybug using natural predators has been established, resistance breeding remains an important means of control. Understanding plant responses to insect herbivory, by determining and identifying differentially expressed genes (DEGs), is a vital step towards the understanding of molecular mechanisms of defence responses in plants and the development of resistant cultivars by gene editing. Morphological and molecular analysis confirmed the mealybug identity as Phenacoccus manihoti (Matile-Ferrero). The transcriptome response of the green mite resistant cassava genotype AR23.1 was compared to P40/1 with no known resistance at 24 and 72 hours of mealybug infestation compared to non-infested mock. A total of 301 and 206 genes were differentially expressed at 24 and 72 of mealybug infestation for AR23.1 and P40/1 genotypes respectively, using a log2 fold change and P-value |
| Keywords: | "Animals Disease Resistance/*genetics Gene Expression Regulation, Plant/genetics Genotype Hemiptera/pathogenicity Manihot/*genetics/growth & development/parasitology *Pest Control, Biological Plant Breeding Plant Diseases/genetics/parasitology Signal Trans;" |
| Notes: | "MedlineRauwane, Molemi E Odeny, Damaris A Millar, Ian Rey, Chrissie Rees, Jasper eng Research Support, Non-U.S. Gov't 2018/08/23 PLoS One. 2018 Aug 22; 13(8):e0202541. doi: 10.1371/journal.pone.0202541. eCollection 2018" |
