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J Anal Toxicol

Title:		Headspace in-tube extraction gas chromatography-mass spectrometry for the analysis of hydroxylic methyl-derivatized and volatile organic compounds in blood and urine
Author(s):		Rasanen I; Viinamaki J; Vuori E; Ojanpera I;
Address:		"Department of Forensic Medicine, Kytosuontie 11,University of Helsinki, Finland"
Journal Title:		J Anal Toxicol
Year:		2010
Volume:		34
Issue:		3
Page Number:		113 - 121
DOI:		10.1093/jat/34.3.113
ISSN/ISBN:		1945-2403 (Electronic) 0146-4760 (Linking)
Abstract:		"A novel headspace in-tube extraction gas chromatography-mass spectrometry (ITEX-GC-MS) approach was developed for broad-scale analysis of low molecular weight organic compounds in blood and/or urine. One sample was analyzed following in-vial derivatization with dimethyl sulfate for ethylene glycol (EG), glycolic acid (GA), formic acid (FA), other hydroxylic compounds, and another sample for underivatized volatile organic compounds. Tenax adsorbent resin was used in the microtrap, and a porous layer, open tubular GC capillary column was used for separation. MS was operated in the full-scan mode, identification was based on the Automated Mass Spectral Deconvolution and Identification System, and quantification was based on extracted ions. The limits of quantification for EG, GA, and FA in blood were 10, 50, and 30 mg/L, respectively, and the expanded uncertainties of measurement were 20%, 16%, and 14%, respectively. The procedure allowed for the first time the inclusion of EG and GA as their methyl derivatives within a quantitative HS analysis. The ITEX method described here was more sensitive for analysis of volatile organic compounds than the corresponding static headspace analysis as demonstrated for 11 representative compounds"
Keywords:		Alcohols/*analysis/blood/chemistry/urine Animals Carboxylic Acids/*analysis/blood/chemistry/urine Cattle Chemical Fractionation/*methods Ethylene Glycol/analysis/blood/chemistry/urine Formates/analysis/blood/chemistry/urine Gas Chromatography-Mass Spectro;
Notes:		"MedlineRasanen, Ilpo Viinamaki, Jenni Vuori, Erkki Ojanpera, Ilkka eng England 2010/04/22 J Anal Toxicol. 2010 Apr; 34(3):113-21. doi: 10.1093/jat/34.3.113"