| Title: | Co-cultivation of Mortierellaceae with Pseudomonas helmanticensis affects both their growth and volatilome |
| Author(s): | Probst M; Telagathoti A; Siewert B; Khomenko I; Betta E; Biasioli F; Peintner U; |
| Address: | "Department of Microbiology, Universitat Innsbruck, Technikerstrasse 25, 6020, Innsbruck, Austria. probst.maraike@gmail.com. Department of Microbiology, Universitat Innsbruck, Technikerstrasse 25, 6020, Innsbruck, Austria. Institute of Pharmacy, Center for Chemistry and Biomedicine, Center for Molecular Biosciences Innsbruck (CMBI), Universitat Innsbruck, Innrain 80 - 82/IV, 6020, Innsbruck, Austria. Research and Innovation Centre, Fondazione Edmund Mach, Via Edmund Mach 1, 38010, San Michele all'Adige, Italy" |
| DOI: | 10.1038/s41598-023-29134-6 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Volatile organic compounds (VOCs) might mediate microbial interactions, especially in spatially structured environments, such as soil. However, the variety and specificity of VOC production are poorly understood. Here, we studied 25 Mortierellaceae strains belonging to the genera Linnemannia and Entomortierella in both pure and co-culture with Pseudomonas helmanticensis under laboratory conditions. We analysed both the fungal growth depending on co-cultivation and the cultures' volatilomes applying proton-transfer-reaction time-of-flight and gas chromatography-mass spectrometry (PTR-ToF-MS and GC-MS). In a strain-specific manner, we found the fungi's radial growth rate and colony morphology affected by the presence of P. helmanticensis. The fungus seemed to generally reduce the bacterial growth. The volatilomes of the fungal and bacterial pure and co-cultures were diverse. While the fungi frequently consumed VOCs, P. helmanticensis produced a higher diversity and amount of VOCs than any fungal strain. Our results support that both the pure and co-culture volatilomes are taxonomically conserved. Taken together, our data supports the relevance of VOCs in Mortierellaceae-P. helmanticensis interaction. We also discuss individual VOCs that appear relevant in the interaction" |
| Keywords: | Gas Chromatography-Mass Spectrometry/methods *Fungi *Volatile Organic Compounds/analysis Protons; |
| Notes: | "MedlineProbst, Maraike Telagathoti, Anusha Siewert, Bianka Khomenko, Iuliia Betta, Emanuela Biasioli, Franco Peintner, Ursula eng Research Support, Non-U.S. Gov't England 2023/02/08 Sci Rep. 2023 Feb 7; 13(1):2213. doi: 10.1038/s41598-023-29134-6" |
