| Title: | The influence of the essential oil extracted from hops on monolayers and bilayers imitating plant pathogen bacteria membranes |
| Author(s): | Polec K; Barnas B; Kowalska M; Dymek M; Rachwalik R; Sikora E; Biela A; Kobialka M; Wojcik K; Hac-Wydro K; |
| Address: | "Department of Environmental Chemistry, Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387, Krakow, Poland. Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387, Krakow, Poland. Institute of Organic Chemistry and Technology, Cracow University of Technology, Warszawska 24, Krakow, Poland. Department of Cell Biology and Imaging, Institute of Zoology and Biomedical Research, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland; Bionanoscience and Biochemistry Laboratory, Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, 30-387 Krakow, Poland. Department of Cell Biology and Imaging, Institute of Zoology and Biomedical Research, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland. Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland. Department of Environmental Chemistry, Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387, Krakow, Poland. Electronic address: hac@chemia.uj.edu.pl" |
| Journal Title: | Colloids Surf B Biointerfaces |
| DOI: | 10.1016/j.colsurfb.2018.10.047 |
| ISSN/ISBN: | 1873-4367 (Electronic) 0927-7765 (Linking) |
| Abstract: | "Many plant-derived compounds possess antimicrobial, antioxidant and even anticancer activities. Therefore, they are considered as substances that can be used instead of synthetic compounds in various applications. In this work, the essential oil from hop cones was extracted and analyzed, and then its effects on model bacteria membranes were studied to verify whether the hop essential oils could be used as ecological pesticides. The experiments involved surface pressure-area measurements, penetration studies and Brewster angle microscopy (BAM) imaging of lipid monolayers as well as hydrodynamic diameter, zeta potential, steady-state fluorescence anisotropy and Cryo-Transmission Electron Microscopy (cryo-TEM) measurements of liposomes. Finally the bactericidal tests on plant pathogen bacteria Pseudomonas syringae pv. lachrymans PCM 1410 were performed. The obtained results showed that the components of the essential oils from hop cones incorporate into lipid monolayers and bilayers and alter their fluidity. However, the observed effect is determined by the system composition, its condensation and the oil concentration. Interestingly, at a given dose, the effect of the essential oil on membranes was found to stabilize. Moreover, BAM images proved that hop oil prevents the formation of a large fraction of a condensed phase at the interface. Both the studies on model membranes as well as the in vitro tests allow one to conclude that the hop essential oil could likely be considered as the candidate to be used in agriculture as a natural pesticide" |
| Keywords: | "Anti-Bacterial Agents/chemistry/isolation & purification/*pharmacology Cardiolipins/chemistry Humulus/*chemistry Lipid Bilayers/*chemistry Membrane Fluidity/drug effects Microbial Sensitivity Tests Oils, Volatile/chemistry/isolation & purification/*pharma;" |
| Notes: | "MedlinePolec, Karolina Barnas, Beata Kowalska, Magdalena Dymek, Michal Rachwalik, Rafal Sikora, Elzbieta Biela, Artur Kobialka, Michal Wojcik, Kinga Hac-Wydro, Katarzyna eng Netherlands 2018/11/02 Colloids Surf B Biointerfaces. 2019 Jan 1; 173:672-680. doi: 10.1016/j.colsurfb.2018.10.047. Epub 2018 Oct 19" |
