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Chem Senses

Title:		A candidate pheromone receptor and two odorant receptors of the hawkmoth Manduca sexta
Author(s):		Patch HM; Velarde RA; Walden KK; Robertson HM;
Address:		"Department of Entomology, Center for Chemical Ecology, The Pennsylvania State University, University Park, PA 16802, USA. hmpatch@psu.edu"
Journal Title:		Chem Senses
Year:		2009
Volume:		20090202
Issue:		4
Page Number:		305 - 316
DOI:		10.1093/chemse/bjp002
ISSN/ISBN:		1464-3553 (Electronic) 0379-864X (Linking)
Abstract:		"In this study, we cloned and characterized three Manduca sexta odorant receptors (ORs). One receptor is a putative pheromone receptor expressed exclusively in a cell associated with male-specific type-I trichoid sensilla. We describe the results of real-time PCR (RT-PCR) and quantitative real-time PCR (qRT-PCR) experiments that show MsextaOR1 is expressed only in male antennae. In situ hybridization labels a single cell associated with type-1 trichoid sensilla, which houses two neurons that have been previously determined to respond to the major components of the pheromone blend. The second receptor, MsextaOR2, was discovered using degenerate primers designed to conserved motifs of a unique group ORs that share as much as 88% identity. Comparison of RT-PCR, qRT-PCR, and in situ hybridization results with those of ORs in the Drosophila melanogaster Or83b subfamily shows a strong sequence and expression pattern similarity. The third receptor, MsextaOR3, was found by 5'-end sequencing of a normalized and subtracted cDNA library from male M. sexta antennae. RT-PCR and qRT-PCR show that this receptor is expressed only in male and female antennae. These are the first ORs, including a putative pheromone receptor, to be described from M. sexta"
Keywords:		"Animals Female In Situ Hybridization Male Manduca/*genetics Microscopy, Electron, Scanning Phylogeny Receptors, Odorant/classification/*genetics Receptors, Pheromone/classification/*genetics Reverse Transcriptase Polymerase Chain Reaction;"
Notes:		"MedlinePatch, Harland M Velarde, Rodrigo A Walden, Kimberly K O Robertson, Hugh M eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2009/02/04 Chem Senses. 2009 May; 34(4):305-16. doi: 10.1093/chemse/bjp002. Epub 2009 Feb 2"