| Title: | Analysis of Volatile Markers for Virgin Olive Oil Aroma Defects by SPME-GC/FID: Possible Sources of Incorrect Data |
| Author(s): | Oliver-Pozo C; Aparicio-Ruiz R; Romero I; Garcia-Gonzalez DL; |
| Address: | "Instituto de la Grasa, (CSIC) , Campus University Pablo de Olavide, Building 46, Ctra. de Utrera, km. 1, E-41013 Sevilla, Spain" |
| ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Linking) |
| Abstract: | "The need to explain virgin olive oil (VOO) aroma descriptors by means of volatiles has raised interest in applying analytical techniques for trapping and quantitating volatiles. Static headspace sampling with solid phase microextraction (SPME) as trapping material is one of the most applied solutions for analyzing volatiles. The use of an internal standard and the determination of the response factors of the main volatiles seem to guarantee the correct determination of volatile concentrations in VOOs by SPME-GC/FID. This paper, however, shows that the competition phenomena between volatiles in their adsorption to the SPME fiber, inherent in static headspace sampling, may affect the quantitation. These phenomena are more noticeable in the particular case of highly odorant matrices, such as rancid and vinegary VOOs with high intensity of defect. The competition phenomena can modify the measurement sensitivity, which can be observed in volatile quantitation as well as in the recording of internal standard areas in different matrices. This paper analyzes the bias of the peak areas and concentrations of those volatiles that are markers for each sensory defect of VOOs (rancid, vinegary, musty, and fusty) when the intensity and complexity of aroma are increased. Of the 17 volatile markers studied in this work, 10 presented some anomalies in the quantitation in highly odorant matrices due the competition phenomena. However, quantitation was not affected in the concentration ranges at which each volatile marker is typically found in the defective oils they were characteristic of, validating their use as markers" |
| Keywords: | Adult Female Gas Chromatography-Mass Spectrometry/*methods/standards Humans Male Odorants/analysis Olive Oil/*chemistry Solid Phase Microextraction/instrumentation/*methods/standards Volatile Organic Compounds/*chemistry/isolation & purification Volatiliz; |
| Notes: | "MedlineOliver-Pozo, Celia Aparicio-Ruiz, Ramon Romero, Inmaculada Garcia-Gonzalez, Diego L eng Research Support, Non-U.S. Gov't 2015/11/17 J Agric Food Chem. 2015 Dec 9; 63(48):10477-83. doi: 10.1021/acs.jafc.5b03986. Epub 2015 Nov 20" |
