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Chem Senses

Title:		Detection and removal of an artefact fatty acid from the binding site of recombinant Bombyx mori pheromone-binding protein
Author(s):		Oldham NJ; Krieger J; Breer H; Svatos A;
Address:		"Max-Planck-Institute for Chemical Ecology, Carl-Zeiss-Promenade 10, D-07745 Jena, Germany. oldham@ice.mpg.de"
Journal Title:		Chem Senses
Year:		2001
Volume:		26
Issue:		5
Page Number:		529 - 531
DOI:		10.1093/chemse/26.5.529
ISSN/ISBN:		0379-864X (Print) 0379-864X (Linking)
Abstract:		"Recombinant Bombyx mori pheromone-binding protein (PBP), purified from an Escherichia coli expression system, has been found to contain (11Z)-octadecenoic acid (cis-vaccenic acid) as an artefact ligand. An efficient delipidation procedure is described to overcome what would appear to be a general problem with recombinant lepidopteran PBPs"
Keywords:		Animals Binding Sites/physiology Bombyx/*chemistry/physiology Carrier Proteins/*chemistry/metabolism Escherichia coli/genetics Insect Proteins/chemistry Intercellular Signaling Peptides and Proteins Recombinant Proteins/*chemistry/genetics/isolation & pur;
Notes:		"MedlineOldham, N J Krieger, J Breer, H Svatos, A eng England 2001/06/22 Chem Senses. 2001 Jun; 26(5):529-31. doi: 10.1093/chemse/26.5.529"