| Title: | Characterization of terpene synthase genes potentially involved in black fig fly (Silba adipata) interactions with Ficus carica |
| Author(s): | Nawade B; Shaltiel-Harpaz L; Yahyaa M; Kabaha A; Kedoshim R; Bosamia TC; Ibdah M; |
| Address: | "Newe Yaar Research Center, Agricultural Research Organization, P. O. Box 1021, Ramat Yishay, 30095, Israel. Tel Hai College, Upper Galilee, 12210, Israel; Migal Galilee Research Institute, P.O. Box 831, Kiryat Shmona, 11016, Israel. Migal Galilee Research Institute, P.O. Box 831, Kiryat Shmona, 11016, Israel. ICAR-Directorate of Groundnut Research, P.O. Box 362001, Junagadh, Gujarat, India. Newe Yaar Research Center, Agricultural Research Organization, P. O. Box 1021, Ramat Yishay, 30095, Israel. Electronic address: mwafaq@volcani.agri.gov.il" |
| DOI: | 10.1016/j.plantsci.2020.110549 |
| ISSN/ISBN: | 1873-2259 (Electronic) 0168-9452 (Linking) |
| Abstract: | "The black fig fly (Silba adipata) is one of the major pests of figs worldwide. This study investigated the effect of pollination on black fig fly infestation and volatile emission during fruit development of facultative parthenocarpic Ficus carica. The results from in-field oviposition preference of black fig fly, olfactory analysis, and fruit volatile profiles indicate that the black fig fly gave a strong preference to unpollinated figs that showed higher emissions of volatile organic compounds. Terpenes are known to be important compounds determining many insect-plant interactions, so we report a transcriptome-based identification and functional characterization of a terpene synthase (TPS) gene family in F. carica. The protein expression in Escherichia coli of eight terpene synthases (TPSs) revealed that three were monoterpene synthases belonging to the TPS-b clade, with FcTPS6 catalyzing the formation of 1,8-cineole while the other two converted GPP into linalool. Four sesquiterpene synthases from the TPS-a clade catalyze the formation of germacrene D (FcTPS1), E-beta-caryophyllene (FcTPS2), cadinene (FcTPS3) and delta-elemene (FcTPS5) while one sesquiterpene synthase FcTPS4 from the TPS-b clade showed nerolidol synthase activity. Most of the enzymatic products closely matched the volatile terpenes emitted from fig fruits and all the genes were expressed during fruit development. This study provides new insights into fig-insect interactions and understanding the molecular mechanisms of terpene biosynthesis and could provide the foundations for sustainable pest management strategies" |
| Keywords: | Animals Diptera/*physiology Female Ficus/*growth & development Fruit/growth & development *Olfactory Perception *Oviposition *Pollination Volatile Organic Compounds/*metabolism Black fig fly Ficus carica Fig Insect-plant interaction Oviposition Pollinatio; |
| Notes: | "MedlineNawade, Bhagwat Shaltiel-Harpaz, Liora Yahyaa, Mosaab Kabaha, Anas Kedoshim, Rika Bosamia, Tejas C Ibdah, Mwafaq eng Ireland 2020/08/11 Plant Sci. 2020 Sep; 298:110549. doi: 10.1016/j.plantsci.2020.110549. Epub 2020 Jun 16" |
