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J Microbiol Biotechnol

Title:		Anti-Inflammatory Activity of Oligomeric Proanthocyanidins Via Inhibition of NF-kappaB and MAPK in LPS-Stimulated MAC-T Cells
Author(s):		Ma X; Wang R; Yu S; Lu G; Yu Y; Jiang C;
Address:		"Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, College of Animal Science and Technology, Southwest University, Chonqing 400715, P.R. China"
Journal Title:		J Microbiol Biotechnol
Year:		2020
Volume:		30
Issue:		10
Page Number:		1458 - 1466
DOI:		10.4014/jmb.2006.06030
ISSN/ISBN:		1738-8872 (Electronic) 1017-7825 (Print) 1017-7825 (Linking)
Abstract:		"Oligomeric proanthocyanidins (OPCs), classified as condensed tannins, have significant antioxidation, anti-inflammation and anti-cancer effects. This study was performed to investigate the anti-inflammatory effects of OPCs and the mechanism underlying these effects in lipopolysaccharide (LPS)-stimulated bovine mammary epithelial cells (MAC-T). Real-time PCR and ELISA assays indicated that OPC treatment at 1, 3 and 5 mug/ml significantly reduced the mRNA and protein, respectively, of oxidant indicators cyclooxygenase-2 (COX-2) (p < 0.05) and inducible nitric oxide synthase (iNOS) (p < 0.01) as well as inflammation cytokines interleukin (IL)-6 (p < 0.01), IL-1beta (p < 0.01) and tumor necrosis factor-alpha (TNF-alpha) (p < 0.05) in LPS-induced MAC-T cells. Moreover, OPCs downregulated LPSinduced phosphorylation of p65 and inhibitor of nuclear factor kappa B (NF-kappaB) (IkappaB) in the NF-kappaB signaling pathway (p < 0.01), and they inhibited p65 translocation from the cytoplasm to the nucleus as revealed by immunofluorescence test and western blot. Additionally, OPCs decreased phosphorylation of p38, extracellular signal regulated kinase and c-jun NH(2)-terminal kinase in the MAPK signaling pathway (p < 0.01). In conclusion, the anti-inflammatory and antioxidant activities of OPCs involve NF-kappaB and MAPK signaling pathways, thus inhibiting expression of pro-inflammatory factors and oxidation indicators. These findings provide novel experimental evidence for the further practical application of OPCs in prevention and treatment of bovine mastitis"
Keywords:		Animals Anti-Inflammatory Agents/*pharmacology Antioxidants/*pharmacology Cattle Cell Survival/drug effects Cyclooxygenase 2/metabolism Epithelial Cells/drug effects/metabolism Inflammation/therapy Interleukin-1beta/metabolism Interleukin-6/metabolism Lip;
Notes:		"MedlineMa, Xiao Wang, Ruihong Yu, Shitian Lu, Guicong Yu, Yongxiong Jiang, Caode eng Korea (South) 2020/09/03 J Microbiol Biotechnol. 2020 Oct 28; 30(10):1458-1466. doi: 10.4014/jmb.2006.06030"