| Title: | Connectivity-based ab initio phasing: from low resolution to a secondary structure |
| Author(s): | Lunina N; Lunin VY; Urzhumtsev A; |
| Address: | "Institute of Mathematical Problems of Biology, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia" |
| Journal Title: | Acta Crystallogr D Biol Crystallogr |
| DOI: | 10.1107/s0907444903015154 |
| ISSN/ISBN: | 0907-4449 (Print) 0907-4449 (Linking) |
| Abstract: | "The connectivity-based phasing method currently allows ab initio determination of phases for several hundred reflections. In the case of large macromolecular crystals, these reflections correspond to a very low resolution and the structural information deduced essentially consists of the molecular packing and an approximate molecular envelope. However, when the unit cell is relatively small, such a phasing procedure can produce phases such that secondary-structure elements can be identified in the corresponding maps. In the case of the pheromone Er-1, all three alpha-helices present are seen in the ab initio phased maps. In the case of protein G, not only the alpha-helix but also some individual beta-strands are distinguishable" |
| Keywords: | "Cluster Analysis Crystallization Crystallography, X-Ray/*methods Fourier Analysis GTP-Binding Proteins/chemistry Models, Chemical Models, Molecular Pheromones/chemistry Protein Structure, Secondary Proteins/*chemistry;" |
| Notes: | "MedlineLunina, Natalia Lunin, Vladimir Y Urzhumtsev, Alexandre eng Comparative Study Research Support, Non-U.S. Gov't 2003/09/23 Acta Crystallogr D Biol Crystallogr. 2003 Oct; 59(Pt 10):1702-15. doi: 10.1107/s0907444903015154. Epub 2003 Sep 19" |
