| Title: | Functional characterization of pheromone receptors in the moth Athetis dissimilis (Lepidoptera: Noctuidae) |
| Author(s): | Liu XL; Sun SJ; Khuhro SA; Elzaki MEA; Yan Q; Dong SL; |
| Address: | "Education Ministry Key Laboratory of Integrated Management of Crop Disease and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China. Institute of Crop Resistance and Chemical Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Education Ministry Key Laboratory of Integrated Management of Crop Disease and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: sldong@njau.edu.cn" |
| DOI: | 10.1016/j.pestbp.2019.04.011 |
| ISSN/ISBN: | 1095-9939 (Electronic) 0048-3575 (Linking) |
| Abstract: | "Sex pheromones are crucial for communication between females and males in moths, and pheromone receptors (PRs) play a key role in peripheral coding of sex pheromones. During the last decade, many PR candidates have been identified based on transcriptome sequencing and bioinformatic analysis, but their detailed functions remain mostly unknown. Here, focusing on four PR candidates of Athetis dissimilis (AdisOR1, AdisOR6, AdisOR11 and AdisOR14) identified in a previous study, we first cloned the full-length cDNAs and determined the tissue expression profiles by quantitative real-time PCR (qPCR). The results revealed that expression of three of these genes were male antennae-specific, while AdisOR11 was similar in expression between male and female antennae. Furthermore, the expression level of AdisOR1 was much higher than those of the other three genes. Then, functional analysis was conducted using Xenopus oocyte system. AdisOR1 responded strongly to the sex pheromone component Z9-14:OH and the potential pheromone component Z9,E12-14:OH, suggesting its important role in the sex pheromone perception; AdisOR14 showed specificity for Z9,E12-14:OH; while AdisOR6 and AdisOR11 did not respond to any of the pheromone components and analogs tested. Taken together, this study contributes to elucidate the molecular mechanism of sex pheromone reception and provides potential targets for development of OR based pest control techniques in A. dissimilis" |
| Keywords: | "Animals Female Insect Proteins/genetics/*metabolism Lepidoptera/genetics/*metabolism Male Pheromones/metabolism Real-Time Polymerase Chain Reaction Receptors, Pheromone/genetics/*metabolism Athetis dissimilis Pheromone receptor Sex pheromone Xenopus oocyt;" |
| Notes: | "MedlineLiu, Xiao-Long Sun, Si-Jie Khuhro, Sajjad Ali Elzaki, Mohammed Esmail Abdalla Yan, Qi Dong, Shuang-Lin eng 2019/08/06 Pestic Biochem Physiol. 2019 Jul; 158:69-76. doi: 10.1016/j.pestbp.2019.04.011. Epub 2019 Apr 22" |
