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Anal Bioanal Chem

Title:		HPLC-CAD as a supplementary method for the quantification of related structure impurities for the purity assessment of organic CRMs
Author(s):		Liu S; Lu B; Peng Z; Liu C; Liu Y; Jiao H; Wu D; Li P; Zhao X; Song S;
Address:		"National Institute of Metrology, China, Beijing, 100029, China. Tianjin University of Technology, Tianjin, 300384, China. Guangxi Zhuang Autonomous Region Ecological and Environmental Monitoring Centre, Nanning, 530028, China. Tianjin Eco-Environmental Monitoring Center, Tianjin, 300191, China. hjwudan123@163.com. Department for Evolutionary Ecology and Environmental Toxicology, Goethe University, Frankfurt Am Main, 60438, Germany. National Institute of Metrology, China, Beijing, 100029, China. songsj@nim.ac.cn. Tianjin University of Technology, Tianjin, 300384, China. songsj@nim.ac.cn"
Journal Title:		Anal Bioanal Chem
Year:		2023
Volume:		20230508
Issue:		17
Page Number:		3375 - 3384
DOI:		10.1007/s00216-023-04719-2
ISSN/ISBN:		1618-2650 (Electronic) 1618-2642 (Linking)
Abstract:		"In organic purity assessment, chromatography separation with a suitable detector is required. Diode array detection (DAD) has been a widely used technique for high-performance liquid chromatography (HPLC) analyses, but its application is limited to compounds with sufficient UV chromophores. Charged aerosol detector (CAD), as a mass-dependent detector, is advantageous for providing a nearly uniform response for analytes, regardless of their structures. In this study, 11 non-volatile compounds with/without UV chromophores were analyzed by CAD using continuous direct injection mode. The RSDs of CAD responses were within 17%. For saccharides and bisphenols, especially, the RSDs were lower (2.12% and 8.14%, respectively). Since bisphenols exist in UV chromophores, their HPLC-DAD responses were studied and compared with CAD responses, with CAD showing a more uniform response. Besides, the key parameters of HPLC-CAD were optimized and the developed method was verified using a Certified Reference Material (CRM, dulcitol, GBW06144). The area normalization result of dulcitol measured by HPLC-CAD was 99.89% +/- 0.02% (n = 6), consistent with the certified value of 99.8% +/- 0.2% (k = 2). The result of this work indicated that the HPLC-CAD method could be a good complementary tool to traditional techniques for the purity assessment of organic compounds, especially for compounds lacking UV chromophores"
Keywords:		"Chromatography, High Pressure Liquid/methods *Phenols/analysis Aerosols/analysis *Benzhydryl Compounds/analysis Charged aerosol detector Purity assessment Related structure impurity Response uniformity;"
Notes:		"PubMed-not-MEDLINELiu, Si Lu, Boling Peng, Zijuan Liu, Chunyu Liu, Yuhui Jiao, Hui Wu, Dan Li, Penghui Zhao, Xingchen Song, Shanjun eng AKY1720/Fundamental Research Funds of National Institute of Metrology, China/ 21806158/National Natural Science Foundation of China/ Germany 2023/05/08 Anal Bioanal Chem. 2023 Jul; 415(17):3375-3384. doi: 10.1007/s00216-023-04719-2. Epub 2023 May 8"