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Int J Mol Sci

Title:		Insights into the Cytochrome P450 Monooxygenase Superfamily in Osmanthus fragrans and the Role of OfCYP142 in Linalool Synthesis
Author(s):		Liu J; Hu H; Shen H; Tian Q; Ding W; Yang X; Wang L; Yue Y;
Address:		"Key Laboratory of Landscape Architecture, Jiangsu Province, College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China. Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China"
Journal Title:		Int J Mol Sci
Year:		2022
Volume:		20221012
Issue:		20
Page Number:		-
DOI:		10.3390/ijms232012150
ISSN/ISBN:		1422-0067 (Electronic) 1422-0067 (Linking)
Abstract:		"Osmanthus fragrans flowers have long been used as raw materials in food, tea, beverage, and perfume industries due to their attractive and strong fragrance. The P450 superfamily proteins have been reported to widely participate in the synthesis of plant floral volatile organic compounds (VOCs). To investigate the potential functions of P450 superfamily proteins in the fragrance synthesis of O. fragrans, we investigated the P450 superfamily genome wide. A total of 276 P450 genes were identified belonging to 40 families. The RNA-seq data suggested that many OfCYP genes were preferentially expressed in the flower or other organs, and some were also induced by multiple abiotic stresses. The expression patterns of seven flower-preferentially expressed OfCYPs during the five different flower aroma content stages were further explored using quantitative real-time PCR, showing that the CYP94C subfamily member OfCYP142 had the highest positive correlation with linalool synthesis gene OfTPS2. The transient expression of OfCYP142 in O. fragrans petals suggested that OfCYP142 can increase the content of linalool, an important VOC of the O. fragrans floral aroma, and a similar result was also obtained in flowers of OfCYP142 transgenic tobacco. Combined with RNA-seq data of the transiently transformed O. fragrans petals, we found that the biosynthesis pathway of secondary metabolites was significantly enriched, and many 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway genes were also upregulated. This evidence indicated that the OfCYP proteins may play critical roles in the flower development and abiotic response of O. fragrans, and that OfCYP142 can participate in linalool synthesis. This study provides valuable information about the functions of P450 genes and a valuable guide for studying further functions of OfCYPs in promoting fragrance biosynthesis of ornamental plants"
Keywords:		Humans *Volatile Organic Compounds *Perfume *Oleaceae/genetics Flowers/genetics Cytochrome P-450 Enzyme System/genetics Tea Osmanthus fragrans cytochrome P450 monooxygenase fragrance linalool;
Notes:		"MedlineLiu, Jiawei Hu, Hongmin Shen, Huimin Tian, Qingyin Ding, Wenjie Yang, Xiulian Wang, Lianggui Yue, Yuanzheng eng 32071828/National Natural Science Foundation of China/ 31870695/National Natural Science Foundation of China/ KYCX21_0929/Postgraduate Research & Practice Innovation Program of Jiangsu Province/ Switzerland 2022/10/28 Int J Mol Sci. 2022 Oct 12; 23(20):12150. doi: 10.3390/ijms232012150"