| Title: | Formation of volatiles in response to tea green leafhopper (Empoasca onukii Matsuda) herbivory in tea plants: a multi-omics study |
| Author(s): | Liu H; Li S; Xiao G; Wang Q; |
| Address: | "College of Light Industry and Food, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China. College of Light Industry and Food, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China. zhongkai_wq@126.com" |
| DOI: | 10.1007/s00299-021-02674-9 |
| ISSN/ISBN: | 1432-203X (Electronic) 0721-7714 (Linking) |
| Abstract: | "Combined transcriptome and metabolome analysis of fresh leaf infestation by tea green leafhoppers (Empoasca (Matsumurasca) onukii Matsuda) suggests roles for alternative pre-mRNA splicing and mRNAs in the regulation of aroma formation in tea plants. Oriental Beauty is a high-grade, oolong tea with a pronounced honey-like aroma and rich ripe fruit flavor that develops primarily as a result of the infestation of the fresh leaves by tea green leafhoppers (Empoasca (Matsumurasca) onukii Matsuda). Here, we used PacBio Iso-Seq and RNA-seq analyses to determine the full-length transcripts and gene expression profiles of fresh tea leaves in response to E. (M.) onukii herbivory. We investigated the relationship between RNA-seq, tea metabolites, and aroma response mechanisms in leaves infested by leafhoppers. We found 3644 differentially expressed genes, of which 2552 were up- and 1092 were down-regulated. A total of 49,913 alternative splicing events were predicted, including 324 differential AS events. Moreover, 3105 differentially expressed transcripts were also identified, of which 2295 were up- and 810 were down-regulated. The characterization of expression patterns of the key gene transcript isoforms involved in the aroma formation pathways identified 130 differentially expressed metabolites, 97 of which were up- and 33 were down-regulated. Two key aroma compounds (phenylacetaldehyde and 4-hydroxybenzaldehyde) were highly correlated with genes of the aroma formation pathways. Our results revealed that pre-mRNA AS plays a crucial role in the metabolic regulation surrounding aroma formation under leafhopper herbivory in tea plants" |
| Keywords: | "Alternative Splicing Animals Camellia sinensis/*physiology Carotenoids/metabolism Fatty Acids/genetics/metabolism Gene Expression Profiling Gene Expression Regulation, Plant *Hemiptera/physiology Metabolome Plant Leaves/*physiology Terpenes/metabolism Vol;" |
| Notes: | "MedlineLiu, Huifan Li, Sufen Xiao, Gengsheng Wang, Qin eng KA1901003/Targeted Poverty Alleviation and Rural Revitalization Project of Agricultural Science and Technology Commissioner of Guangdong Province/ D11920715/Provincial Modern Agricultural Industrial Park Expert Service Team Docking Service of Xinfeng Country/ Germany 2021/02/23 Plant Cell Rep. 2021 Apr; 40(4):753-766. doi: 10.1007/s00299-021-02674-9. Epub 2021 Feb 22" |
