| Title: | Cloning and functional analysis of three aphid alarm pheromone genes from German chamomile (Matricaria chamomilla L.) |
| Author(s): | Ling C; Zheng L; Yu X; Wang H; Wang C; Wu H; Zhang J; Yao P; Tai Y; Yuan Y; |
| Address: | "School of Life Science, Anhui Agricultural University, Hefei, China. School of Life Science, Anhui Agricultural University, Hefei, China. Electronic address: taiyuling1102@126.com. School of Life Science, Anhui Agricultural University, Hefei, China. Electronic address: zhiwuxue239@163.com" |
| DOI: | 10.1016/j.plantsci.2020.110463 |
| ISSN/ISBN: | 1873-2259 (Electronic) 0168-9452 (Linking) |
| Abstract: | "German chamomile (Matricaria chamomilla L.) is one of the most ancient medicinal species in the world and terpenoids from their flowers have important medicinal value. We cloned three sesquiterpene synthase genes, McGDS1, McGDS2 and McGDS3, and performed sequence alignment and phylogenetic analysis. The encoded proteins possess three conserved structural features: an RRxxxxxxxxW motif, an RxR motif, and a DDxxD motif. McGDS1, McGDS2 and McGDS3 were confirmed to be (E)-farnesene synthase, germacrene D synthase, and germacrene A synthase, respectively. Subcellular localization revealed diffuse GFP reporter-gene signals in the cytoplasm and nucleus. qPCR indicated that McGDS1, McGDS2 and McGDS3, were more highly expressed in young flowers than in old flowers and the expression was highly correlated with amounts of the end-product essential oils ((E)-beta-farnesene, germacrene D and beta-elemene), with coefficients of 0.76, 0.83 and 0.68, respectively. We also established a transformation system for chamomile hairy roots. The overexpression of McGDS1, McGDS2 and McGDS3 resulted in gamma-muurolene accumulation in hairy roots. The activity of three aphid alarm pheromones here forms the molecular basis for the study of the biosynthesis and regulation of volatile terpenes. Transformation of chamomile hairy roots provides a simple system in which to study terpene biosynthesis in chamomile" |
| Keywords: | "Animals Aphids Chamomile/metabolism Flowers/genetics/metabolism Matricaria/genetics/*metabolism Phylogeny Plant Proteins/genetics/metabolism Plant Roots/genetics/metabolism Polymerase Chain Reaction Sesquiterpenes/metabolism Sesquiterpenes, Germacrane/met;" |
| Notes: | "MedlineLing, Chengcheng Zheng, Lujie Yu, Xiaorui Wang, Huanhuan Wang, Chengxiang Wu, Haiyan Zhang, Jie Yao, Ping Tai, Yuling Yuan, Yi eng Ireland 2020/04/03 Plant Sci. 2020 May; 294:110463. doi: 10.1016/j.plantsci.2020.110463. Epub 2020 Mar 6" |
