| Title: | Combined mass spectrometry-guided genome mining and virtual screening for acaricidal activity in secondary metabolites of Bacillus velezensis W1 |
| Author(s): | Li X; Munir S; Xu Y; Wang Y; He Y; |
| Address: | "College of Science, Yunnan Agricultural University Kunming 650201 China lixingyu@ynau.edu.cn. Department of Chemistry, Cleveland State University Cleveland OH 44115 USA. State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan, Yunnan Agricultural University Kunming 650201 Yunnan China ynfh2007@163.com. Key Laboratory of Economic Plants and Biotechnology, Chinese Academy of Sciences Kunming 650201 China" |
| ISSN/ISBN: | 2046-2069 (Electronic) 2046-2069 (Linking) |
| Abstract: | "A comprehensive analytic strategy was performed to study the acaricidal activity ingredients of Bacillus velezensis W1, a strain for biological control of Tetranychus urticae. Through genome mining, 14 biosynthetic gene clusters were identified, which encode secondary metabolites, and these were further confirmed by MALDI-TOF-MS or LC-ESI-MS/MS, including bacillomycin D C13-C17, macrolactin A, 7-O-malonyl-macrolactin A, surfactin C14, and surfactin C15. Moreover, 27 volatile compounds were identified by GC-MS, mainly including cyclodipeptides, alkanes, organic acids, and esters. Finally, 43 compounds identified from W1 were used in the virtual screening of acaricidal activity. The results showed that 16 compounds, including cyclodipeptides, bacillomycins, macrolactins, and surfactins, have acaricidal potential. This work provides a base for studying the mechanism of acaricidal action of B. velezensis W1 and a comprehensive strategy for the study of active ingredients from biocontrol strains" |
| Notes: | "PubMed-not-MEDLINELi, Xingyu Munir, Shahzad Xu, Yan Wang, Yuehu He, Yueqiu eng England 2022/04/29 RSC Adv. 2021 Jul 21; 11(41):25441-25449. doi: 10.1039/d1ra01326b. eCollection 2021 Jul 19" |
