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BMC Genomics

Title:		Identification of new signalling peptides through a genome-wide survey of 250 fungal secretomes
Author(s):		Le Marquer M; San Clemente H; Roux C; Savelli B; Frei Dit Frey N;
Address:		"Laboratoire de Recherche en Sciences Vegetales, Universite de Toulouse, CNRS, UPS, 24 chemin de Borde Rouge, Auzeville, BP42617, 31326, Castanet Tolosan, France. Laboratoire de Recherche en Sciences Vegetales, Universite de Toulouse, CNRS, UPS, 24 chemin de Borde Rouge, Auzeville, BP42617, 31326, Castanet Tolosan, France. frei-dit-frey@lrsv.ups-tlse.fr"
Journal Title:		BMC Genomics
Year:		2019
Volume:		20190118
Issue:		1
Page Number:		64 -
DOI:		10.1186/s12864-018-5414-2
ISSN/ISBN:		1471-2164 (Electronic) 1471-2164 (Linking)
Abstract:		"BACKGROUND: Many small peptides regulate eukaryotic cell biology. In fungi, some of these peptides are produced after KEX2 protease activity on proteins displaying repetitions of identical or nearly identical motifs. Following this endoprotease activity, peptides are released in the extracellular space. This type of protein maturation is involved in the production of the alpha-type sexual pheromone in Ascomycota. In other cases, this processing allows the production of secreted peptides regulating fungal cell wall structure or acting as mycotoxins. In this work, we report for the first time a genome-wide search of KEX2-processed repeat proteins that we call KEPs. We screened the secreted proteins of 250 fungal species to compare their KEP repertoires with regard to their lifestyle, morphology or lineage. RESULTS: Our analysis points out that nearly all fungi display putative KEPs, suggesting an ancestral origin common to all opisthokonts. As expected, our pipeline identifies mycotoxins but also alpha-type sexual pheromones in Ascomycota that have not been explored so far, and unravels KEP-derived secreted peptides of unknown functions. Some species display an expansion of this class of proteins. Interestingly, we identified conserved KEPs in pathogenic fungi, suggesting a role in virulence. We also identified KEPs in Basidiomycota with striking similarities to Ascomycota alpha-type sexual pheromones, suggesting they may also play alternative roles in unknown signalling processes. CONCLUSIONS: We identified putative, new, unexpected secreted peptides that fall into different functional categories: mycotoxins, hormones, sexual pheromones, or effectors that promote colonization during host-microbe interactions. This wide survey will open new avenues in the field of small-secreted peptides in fungi that are critical regulators of their intimate biology and modulators of their interaction with the environment"
Keywords:		"Amino Acid Sequence Ascomycota/classification/genetics/metabolism Basidiomycota/classification/genetics/metabolism Fungal Proteins/*genetics/metabolism Fungi/classification/*genetics/metabolism Genome, Fungal/*genetics Mating Factor/genetics/metabolism Ph;"
Notes:		"MedlineLe Marquer, Morgane San Clemente, Helene Roux, Christophe Savelli, Bruno Frei Dit Frey, Nicolas eng England 2019/01/20 BMC Genomics. 2019 Jan 18; 20(1):64. doi: 10.1186/s12864-018-5414-2"