| Title: | Natural history-guided omics reveals plant defensive chemistry against leafhopper pests |
| Author(s): | Bai Y; Yang C; Halitschke R; Paetz C; Kessler D; Burkard K; Gaquerel E; Baldwin IT; Li D; |
| Address: | "Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, D-07745 Jena, Germany. Department of Biosynthesis/NMR, Max Planck Institute for Chemical Ecology, D-07745 Jena, Germany. Institut de Biologie Moleculaire des Plantes du CNRS, Universite de Strasbourg, Strasbourg, France. National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China. CAS-JIC Center of Excellence for Plant and Microbial Sciences (CEPAMS), Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China" |
| ISSN/ISBN: | 1095-9203 (Electronic) 0036-8075 (Linking) |
| Abstract: | "Although much is known about plant traits that function in nonhost resistance against pathogens, little is known about nonhost resistance against herbivores, despite its agricultural importance. Empoasca leafhoppers, serious agricultural pests, identify host plants by eavesdropping on unknown outputs of jasmonate (JA)-mediated signaling. Forward- and reverse-genetics lines of a native tobacco plant were screened in native habitats with native herbivores using high-throughput genomic, transcriptomic, and metabolomic tools to reveal an Empoasca-elicited JA-JAZi module. This module induces an uncharacterized caffeoylputrescine-green leaf volatile compound, catalyzed by a polyphenol oxidase in a Michael addition reaction, which we reconstitute in vitro; engineer in crop plants, where it requires a berberine bridge enzyme-like 2 (BBL2) for its synthesis; and show that it confers resistance to leafhoppers. Natural history-guided forward genetics reveals a conserved nonhost resistance mechanism useful for crop protection" |
| Keywords: | "Animals Biosynthetic Pathways Catechol Oxidase/genetics/metabolism Crops, Agricultural Cyclopentanes/metabolism Genes, Plant *Hemiptera *Herbivory Metabolome Oxylipins/metabolism Plant Leaves/metabolism Synthetic Biology Tobacco/genetics/*metabolism Trans;" |
| Notes: | "MedlineBai, Yuechen Yang, Caiqiong Halitschke, Rayko Paetz, Christian Kessler, Danny Burkard, Konrad Gaquerel, Emmanuel Baldwin, Ian T Li, Dapeng eng Research Support, Non-U.S. Gov't 2022/02/04 Science. 2022 Feb 4; 375(6580):eabm2948. doi: 10.1126/science.abm2948. Epub 2022 Feb 4" |
