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Enzyme Microb Technol

Title:		De novo biosynthesis of linalool from glucose in engineered Escherichia coli
Author(s):		Kong S; Fu X; Li X; Pan H; Guo D;
Address:		"Key Laboratory of Organo-Pharmaceutical Chemistry, Gannan Normal University, Jiangxi Province, Ganzhou 341000, China. Key Laboratory of Organo-Pharmaceutical Chemistry, Gannan Normal University, Jiangxi Province, Ganzhou 341000, China. Electronic address: panhong_813@163.com. Key Laboratory of Organo-Pharmaceutical Chemistry, Gannan Normal University, Jiangxi Province, Ganzhou 341000, China. Electronic address: ggdy3478@163.com"
Journal Title:		Enzyme Microb Technol
Year:		2020
Volume:		20200605
Issue:
Page Number:		109614 -
DOI:		10.1016/j.enzmictec.2020.109614
ISSN/ISBN:		1879-0909 (Electronic) 0141-0229 (Linking)
Abstract:		"Linalool is an important terpenoids of floral scents and has wide applications. In the past, several groups reported on a strategy to establish biosynthesis of linalool in yeast based on co-expression of Saccharomyces cerevisiae farnesyl diphosphate synthase ERG20 and Actinidia arguta linalool synthase LIS. However, ERG20 has both geranyl diphosphate synthase and farnesyl diphosphate synthase activities, which can lead to metabolic flow to farnesyl diphosphate. In this study, a heterologous linalool biosynthesis pathway was constructed in Escherichia coli and showed that using Abies grandis geranyl diphosphate synthase GPPS2 instead of ERG20 can effectively improve linalool biosynthesis. Subsequently, we further improved the biosynthesis of linalool by overexpression of isopentenyl diphosphate isomerase Idi"
Keywords:		Abies/enzymology/genetics Actinidia/enzymology/genetics Acyclic Monoterpenes/*metabolism Biosynthetic Pathways/genetics Carbon-Carbon Double Bond Isomerases/genetics/metabolism Escherichia coli/*genetics/metabolism Escherichia coli Proteins/genetics/metab;
Notes:		"MedlineKong, Sijia Fu, Xiao Li, Xun Pan, Hong Guo, Daoyi eng 2020/09/12 Enzyme Microb Technol. 2020 Oct; 140:109614. doi: 10.1016/j.enzmictec.2020.109614. Epub 2020 Jun 5"