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J Med Entomol

Title:		Identification of genes encoding atypical odorant-binding proteins in Aedes albopictus (Diptera: Culicidae)
Author(s):		Armbruster P; White S; Dzundza J; Crawford J; Zhao X;
Address:		"Department of Biology, Georgetown University, Washington, DC 20057-1229, USA. paa9@georgetown.edu"
Journal Title:		J Med Entomol
Year:		2009
Volume:		46
Issue:		2
Page Number:		271 - 280
DOI:		10.1603/033.046.0211
ISSN/ISBN:		0022-2585 (Print) 0022-2585 (Linking)
Abstract:		"Insect odorant-binding proteins (OBPs) are a diverse gene family that encode proteins thought to function as molecular chaperones by binding semiochemicals and transporting them through the aqueous lymph of insect sensilla. Between 66 and 68 genes have been classified as OBPs in both Anopheles gambiae (Giles) and Aedes aegypti L. based on bioninformatics criteria. We have cloned and sequenced from a subtracted cDNA library three OBPs in Aedes albopcitus (Skuse). BLASTP and phylogenetic analysis of deduced amino acid sequences identified a unique putative ortholog in Ae. aegypti for each Ae. albopictus OBP. Comparison of these putative Ae. aegypti orthologs with the results of previous bioinformatics analyses of OBP genes in Ae. aegypti highlight the potential variability of bioinformatics analyses and suggest that the OBP gene family of Culicids is even more diverse than previously described. Alignment of deduced amino acid sequences and phylogenetic analysis identified the N-terminal region of Culicid OBPs that is associated with aedine-specific diversification. Analysis of tissue-specific expression indicates that two of the Ae. albopictus OBPs are expressed both in preadult stages and in the hemolymph of adults, suggesting that the proteins encoded by these genes may be involved in the transport of hydrophobic ligands in the hemolymph. The other Ae. albopictus OBP is expressed exclusively in antennae and leg, suggesting a chemosensory function. These results are discussed within the context of the evolution and functional diversification of OBPs in mosquitoes"
Keywords:		"Aedes/*genetics/growth & development Amino Acid Sequence Animals DNA, Complementary/chemistry Evolution, Molecular Female Gene Library Genes, Insect *Life Cycle Stages Male Molecular Sequence Data Multigene Family Nucleic Acid Hybridization Receptors, Odo;"
Notes:		"MedlineArmbruster, Peter White, Sheryl Dzundza, John Crawford, Jacob Zhao, Xiumei eng F32-AI09969-02/AI/NIAID NIH HHS/ P20 RR16435/RR/NCRR NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't England 2009/04/09 J Med Entomol. 2009 Mar; 46(2):271-80. doi: 10.1603/033.046.0211"