« Previous Abstract"Behavioural and chemoreceptor cell responses of the tick, Ixodes ricinus, to its own faeces and faecal constituents"    Next AbstractDysregulation of markers of oxidative stress and DNA damage among nail technicians despite low exposure to volatile organic compounds »

Curr Biol

Title:		Long-distance turgor pressure changes induce local activation of plant glutamate receptor-like channels
Author(s):		Grenzi M; Buratti S; Parmagnani AS; Abdel Aziz I; Bernacka-Wojcik I; Resentini F; Simura J; Doccula FG; Alfieri A; Luoni L; Ljung K; Bonza MC; Stavrinidou E; Costa A;
Address:		"Department of Biosciences, University of Milan, via Celoria 26, 20133 Milano, Italy. Laboratory of Organic Electronics, Department of Science and Technology, Linkoping University, 601 74 Norrkoping, Sweden. Umea Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90183 Umea, Sweden. Department of Biosciences, University of Milan, via Celoria 26, 20133 Milano, Italy; Centro Grandi Strumenti, University of Pavia, via Ferrata 9, 27100 Pavia, Italy. Laboratory of Organic Electronics, Department of Science and Technology, Linkoping University, 601 74 Norrkoping, Sweden; Umea Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90183 Umea, Sweden; Wallenberg Wood Science Center, Department of Science and Technology, Linkoping University, Norrkoping, Sweden. Department of Biosciences, University of Milan, via Celoria 26, 20133 Milano, Italy; Institute of Biophysics, National Research Council of Italy (CNR), 20133 Milano, Italy. Electronic address: alex.costa@unimi.it"
Journal Title:		Curr Biol
Year:		2023
Volume:		20230215
Issue:		6
Page Number:		1019 - 1035
DOI:		10.1016/j.cub.2023.01.042
ISSN/ISBN:		1879-0445 (Electronic) 0960-9822 (Linking)
Abstract:		"In Arabidopsis thaliana, local wounding and herbivore feeding provoke leaf-to-leaf propagating Ca(2+) waves that are dependent on the activity of members of the glutamate receptor-like channels (GLRs). In systemic tissues, GLRs are needed to sustain the synthesis of jasmonic acid (JA) with the subsequent activation of JA-dependent signaling response required for the plant acclimation to the perceived stress. Even though the role of GLRs is well established, the mechanism through which they are activated remains unclear. Here, we report that in vivo, the amino-acid-dependent activation of the AtGLR3.3 channel and systemic responses require a functional ligand-binding domain. By combining imaging and genetics, we show that leaf mechanical injury, such as wounds and burns, as well as hypo-osmotic stress in root cells, induces the systemic apoplastic increase of L-glutamate (L-Glu), which is largely independent of AtGLR3.3 that is instead required for systemic cytosolic Ca(2+) elevation. Moreover, by using a bioelectronic approach, we show that the local release of minute concentrations of L-Glu in the leaf lamina fails to induce any long-distance Ca(2+) waves"
Keywords:		"*Arabidopsis Proteins/genetics/metabolism *Arabidopsis/metabolism Receptors, Glutamate/genetics/metabolism Glutamic Acid Pressure Plant Leaves/metabolism Gene Expression Regulation, Plant Arabidopsis thaliana glutamate receptor-like channels implantable b;"
Notes:		"MedlineGrenzi, Matteo Buratti, Stefano Parmagnani, Ambra Selene Abdel Aziz, Ilaria Bernacka-Wojcik, Iwona Resentini, Francesca Simura, Jan Doccula, Fabrizio Gandolfo Alfieri, Andrea Luoni, Laura Ljung, Karin Bonza, Maria Cristina Stavrinidou, Eleni Costa, Alex eng Research Support, Non-U.S. Gov't England 2023/02/17 Curr Biol. 2023 Mar 27; 33(6):1019-1035.e8. doi: 10.1016/j.cub.2023.01.042. Epub 2023 Feb 15"