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Phytochemistry

Title:		"Effect of Monochamus galloprovincialis feeding on Pinus pinaster and Pinus pinea, oleoresin and insect volatiles"
Author(s):		Goncalves E; Figueiredo AC; Barroso JG; Henriques J; Sousa E; Bonifacio L;
Address:		"Centro de Estudos do Ambiente e do Mar (CESAM Lisboa), Faculdade de Ciencias da Universidade de Lisboa (FCUL), Centro de Biotecnologia Vegetal (CBV), Departamento de Biologia Vegetal (DBV), C2, Campo Grande, 1749-016, Lisboa, Portugal. Electronic address: evgoncalves@fc.ul.pt. Centro de Estudos do Ambiente e do Mar (CESAM Lisboa), Faculdade de Ciencias da Universidade de Lisboa (FCUL), Centro de Biotecnologia Vegetal (CBV), Departamento de Biologia Vegetal (DBV), C2, Campo Grande, 1749-016, Lisboa, Portugal. Electronic address: acsf@fc.ul.pt. Centro de Estudos do Ambiente e do Mar (CESAM Lisboa), Faculdade de Ciencias da Universidade de Lisboa (FCUL), Centro de Biotecnologia Vegetal (CBV), Departamento de Biologia Vegetal (DBV), C2, Campo Grande, 1749-016, Lisboa, Portugal. Electronic address: jmbarroso@fc.ul.pt. Unidade Estrategica de Investigacao e Servicos de Sistemas Agrarios e Florestais e Sanidade Vegetal, Instituto Nacional de Investigacao Agraria e Veterinaria (INIAV), Quinta do Marques, 2780-159, Oeiras, Portugal; University of Lisboa, Faculty of Sciences, BioISI - Biosystems & Integrative Sciences Institute, Lisboa, Portugal. Electronic address: joana.henriques@iniav.pt. Unidade Estrategica de Investigacao e Servicos de Sistemas Agrarios e Florestais e Sanidade Vegetal, Instituto Nacional de Investigacao Agraria e Veterinaria (INIAV), Quinta do Marques, 2780-159, Oeiras, Portugal. Electronic address: edmundo.sousa@iniav.pt. Unidade Estrategica de Investigacao e Servicos de Sistemas Agrarios e Florestais e Sanidade Vegetal, Instituto Nacional de Investigacao Agraria e Veterinaria (INIAV), Quinta do Marques, 2780-159, Oeiras, Portugal. Electronic address: luis.bonifacio@iniav.pt"
Journal Title:		Phytochemistry
Year:		2020
Volume:		20191007
Issue:
Page Number:		112159 -
DOI:		10.1016/j.phytochem.2019.112159
ISSN/ISBN:		1873-3700 (Electronic) 0031-9422 (Linking)
Abstract:		"In Portugal, the pine black sawyer Monochamus galloprovincialis is the principal vector of the pinewood nematode, Bursaphelenchus xylophilus, the causal agent of pine wilt disease (PWD), a lethal phyopathogen with major ecological and economic consequences to European forestry. The aim of this study was to determine the influence of M. galloprovincialis feeding on the volatiles emitted by pine trees. This study focused on the pine species which are most relevant to Portugal, that is, Pinus pinaster (maritime pine) and Pinus pinea (stone or umbrella pine), assessing to what extent pine chemotypes might influence feeding by the insect vector. Preliminary evaluation of each maritime pine essential oil allowed recognizing the existence of two main chemotypes (C1 and C2) and absence of chemical variability in P. pinea. Emission of volatiles from pine trees was evaluated before and during 24?ª+h of feeding by a mixed-sex pair of newly emerged, unfed M. galloprovincialis. Volatiles were also collected from the oleoresin released from the feeding wounds as well as from the insects after feeding. Pine volatiles were collected by solid phase microextraction (SPME) and insect volatiles extracted with pentane, and all analysed by gas chromatography-mass spectrometry (GC-MS) and by GC for component identification and quantification, respectively. Of the seventeen emitted volatiles detected in SPME analyses of P. pinaster, beta-pinene, alpha-pinene, beta-caryophyllene, and germacrene D showed the highest average fold increases as a result of M. galloprovincialis feeding. When grouped by P. pinaster chemotype, C1 and C2 groups of trees showed different patterns of responses. beta-Caryophyllene and germacrene D showed the highest fold increase in C1 trees, whereas beta-pinene and alpha-pinene clearly dominated in C2 trees. Likewise, the oleoresin volatiles from C1 trees were dominated by delta-3-carene and/or beta-pinene, whereas alpha-pinene and beta-pinene were the main volatile components from oleoresin of C2 trees. Nine components were detected in P. pinea volatiles, of which limonene showed the highest fold increase as a result of insect feeding. The volatiles collected from the insects after they had fed on P. pinaster included alpha-pinene, beta-pinene, and abietic acid, and by the straight-chain n-alkanes n-C27, n-C29, and n-C25, together with the methyl-branched hydrocarbons 3-meC29, 2-meC28, and 3-meC27. A better understanding of the responses of different P. pinaster chemotypes to feeding by M. galloprovincialis may be helpful in the development of new lures to improve pine sawyer trapping in integrated pest management for control of PWD"
Keywords:		*Animal Feed Animals Coleoptera/*drug effects Gas Chromatography-Mass Spectrometry Pinus/*chemistry Plant Extracts/*chemistry Portugal Solid Phase Microextraction Volatile Organic Compounds/chemistry/isolation & purification/*pharmacology Maritime pine Ol;
Notes:		"MedlineGoncalves, Elsa Figueiredo, A Cristina Barroso, Jose G Henriques, Joana Sousa, Edmundo Bonifacio, Luis eng England 2019/10/11 Phytochemistry. 2020 Jan; 169:112159. doi: 10.1016/j.phytochem.2019.112159. Epub 2019 Oct 7"