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J Biol Chem

Title:		alpha-Factor-mediatd modification of a 32P-labeled protein by MATa cells of Saccharomyces cerevisiae
Author(s):		Finkelstein DB; McAlister L;
Address:
Journal Title:		J Biol Chem
Year:		1981
Volume:		256
Issue:		5
Page Number:		2561 - 2566
DOI:
ISSN/ISBN:		0021-9258 (Print) 0021-9258 (Linking)
Abstract:		"Addition of the polypeptide mating pheromone alpha-factor to haploid MATa cells of Saccharomyces cerevisiae results in the modification of a 32P-labeled protein (P17) with an apparent Mr of 17,000 to a form having an apparent Mr of 17,500 (P17). 32P associated with both P17 and P17 exhibits an unusually rapid rate of turnover. The conversion of P17 to P17 precedes the appearance of morphologically abnormal cells and, in contrast to other responses elicited by this pheromone, this change in apparent molecular weight does not require protein synthesis. Upon removal of alpha-factor, the P17/P17 ratio returns to pretreatment levels"
Keywords:		Animals Kinetics Mating Factor Molecular Weight Mutation Peptides/*physiology Pheromones Phosphoproteins/*metabolism Phosphorus Radioisotopes Radioisotope Dilution Technique Saccharomyces cerevisiae/*metabolism;
Notes:		"MedlineFinkelstein, D B McAlister, L eng 1R01 GM2529/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1981/03/10 J Biol Chem. 1981 Mar 10; 256(5):2561-6"