| Title: | Isolation of CarE genes from the Chinese white pine beetle Dendroctonus armandi (Curculionidae: Scolytinae) and their response to host chemical defense |
| Author(s): | Dai L; Gao H; Ye J; Fu D; Sun Y; Chen H; |
| Address: | "College of Forestry, Northwest A&F University, Yangling, China. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources (South China Agricultural University), Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China" |
| ISSN/ISBN: | 1526-4998 (Electronic) 1526-498X (Linking) |
| Abstract: | "BACKGROUND: Bark beetles rely on detoxifying enzymes to resist the defensive terpenoids of their host trees. Research on carboxylesterases (CarEs) has focused on their multiple functions in the metabolic detoxification of pesticides and plant allelochemicals, drug resistance, and juvenile hormone and pheromone degradation. RESULT: We identified eight new CarE genes in the Chinese white pine beetle (Dendroctonus armandi) and carried out bioinformatics analysis on the deduced full-length amino acid sequences. Differential transcript levels of CarE genes were observed between sexes; within these levels, significant differences were found among the different development stages, and between insects fed on the phloem of Pinus armandi and exposed to five stimuli [(-)-alpha-pinene, (-)-beta-pinene, (+)-3-carene, limonene and turpentine] at 8 and 24 h. CONCLUSION: Transcription levels of CarE genes suggest some relationship with the detoxification of terpenoids released by host trees. The functions of bark beetle esterase are mainly in hydrolyzing the host chemical defense and degrading odorant molecules during host selection and colonization. (c) 2018 Society of Chemical Industry" |
| Keywords: | "Amino Acid Sequence Animals Carboxylic Ester Hydrolases/chemistry/*genetics/metabolism Female Inactivation, Metabolic Insect Proteins/chemistry/*genetics/metabolism Larva/enzymology/genetics/growth & development Male Monoterpenes/*metabolism Phylogeny Pin;" |
| Notes: | "MedlineDai, Lulu Gao, Haiming Ye, Jiaqi Fu, Danyang Sun, Yaya Chen, Hui eng 2017YFD0600104/National Key Research and Development Program of China/ 2017ZDJC-03/Natural Science Basic Research Plan in Shaanxi Province of China/ Z109021640/Fundamental Research Funds for the Central Universities/ England 2018/09/12 Pest Manag Sci. 2019 Apr; 75(4):986-997. doi: 10.1002/ps.5205. Epub 2018 Oct 18" |
