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Front Microbiol

Title:		"The NADPH Oxidases Nox1 and Nox2 Differentially Regulate Volatile Organic Compounds, Fungistatic Activity, Plant Growth Promotion and Nutrient Assimilation in Trichoderma atroviride"
Author(s):		Cruz-Magalhaes V; Nieto-Jacobo MF; van Zijll de Jong E; Rostas M; Padilla-Arizmendi F; Kandula D; Kandula J; Hampton J; Herrera-Estrella A; Steyaert JM; Stewart A; Loguercio LL; Mendoza-Mendoza A;
Address:		"Bio-Protection Research Centre, Lincoln University, Lincoln, New Zealand. Department of Biological Sciences (DCB), State University of Santa Cruz (UESC), Ilheus, Brazil. UGA-LANGEBIO, Cinvestav, Irapuato, Mexico. Lincoln Agritech Ltd., Lincoln, New Zealand. The Foundation for Arable Research (FAR), Christchurch, New Zealand"
Journal Title:		Front Microbiol
Year:		2018
Volume:		20190123
Issue:
Page Number:		3271 -
DOI:		10.3389/fmicb.2018.03271
ISSN/ISBN:		1664-302X (Print) 1664-302X (Electronic) 1664-302X (Linking)
Abstract:		"In eukaryotic systems, membrane-bound NADPH oxidases (Nox) generate reactive oxygen species (ROS) as a part of normal physiological functions. In the soil-borne mycoparasitic and plant facultative symbiont Trichoderma atroviride, Nox1 and the regulator NoxR are involved in differentiation induced by mechanical damage, while the role of Nox2 has not been determined. The knock-out strains Deltanox1, DeltanoxR and Deltanox2 were compared to the parental strain (WT) in their ability to grow and conidiate under a series of stress conditions (osmotic, oxidative, membrane, and cell-wall stresses). All three genes were differentially involved in the stress-response phenotypes. In addition, several interactive experiments with biotic factors (plant seedlings and other fungi) were performed comparing the mutant phenotypes with the WT, which was used as the reference strain. Deltanox1 and DeltanoxR significantly reduced the antagonistic activity of T. atroviride against Rhizoctonia solani and Sclerotinia sclerotiorum in direct confrontation assays, but Deltanox2 showed similar activity to the WT. The Deltanox1, DeltanoxR, and Deltanox2 mutants showed quantitative differences in the emission of several volatile organic compounds (VOCs). The effects of a blend of these volatiles on plant-growth promotion of Arabidopsis thaliana seedlings were determined in closed-chamber experiments. The increase in root and shoot biomass induced by T. atroviride VOCs was significantly lowered by DeltanoxR and Deltanox1, but not by Deltanox2. In terms of fungistatic activity at a distance, Deltanox2 had a significant reduction in this trait against R. solani and S. sclerotiorum, while fungistasis was highly increased by DeltanoxR and Deltanox1. Identification and quantification of individual VOCs in the blends emitted by the strains was performed by GC-MS and the patterns of variation observed for individual volatiles, such as 6-Pentyl-2H-pyran-2-one (6PP-1) and (E)-6-Pent-1-enylpyran-2-one (6PP-2) were consistent with their negative effects in plant-growth promotion and positive effects in fungistasis at a distance. Nox1 and NoxR appear to have a ubiquitous regulatory role of in a variety of developmental and interactive processes in T. atroviride either as positive or negative modulators. Nox2 may also have a role in regulating production of VOCs with fungistatic activity"
Keywords:		Nox1 Nox2 NoxR Trichoderma plant growth promotion reactive oxygen species volatile organic compounds;
Notes:		"PubMed-not-MEDLINECruz-Magalhaes, Valter Nieto-Jacobo, Maria Fernanda van Zijll de Jong, Eline Rostas, Michael Padilla-Arizmendi, Fabiola Kandula, Diwakar Kandula, Janaki Hampton, John Herrera-Estrella, Alfredo Steyaert, Johanna M Stewart, Alison Loguercio, Leandro Lopes Mendoza-Mendoza, Artemio eng Switzerland 2019/02/08 Front Microbiol. 2019 Jan 23; 9:3271. doi: 10.3389/fmicb.2018.03271. eCollection 2018"