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Front Physiol

Title:		Pyruvate Kinase Is Required for Sex Pheromone Biosynthesis in Helicoverpa armigera
Author(s):		Yao S; Zhang Y; Chang Y; Li X; Zhao W; An S;
Address:		"State Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou, China"
Journal Title:		Front Physiol
Year:		2021
Volume:		20210804
Issue:
Page Number:		707389 -
DOI:		10.3389/fphys.2021.707389
ISSN/ISBN:		1664-042X (Print) 1664-042X (Electronic) 1664-042X (Linking)
Abstract:		"Pyruvate kinase (PYK) is a speed-limited enzyme of glycolysis that catalyzes the formation of pyruvate, and plays an important role in acetyl-CoA synthesis. The acetyl-CoA is the precursor of sex pheromone biosynthesis in Helicoverpa armigera. However, the role of PYK in sex pheromone biosynthesis remains elusive. Here, PYK in H. armigera (HaPYK) was found to be highly expressed in the pheromone glands (PGs). The developmental expression profile of HaPYK was consistent with the fluctuation of sex pheromone release. Function analysis revealed that the knockdown of HaPYK led to a decrease in the levels of pyruvic acid and acetyl-CoA in PGs, which in turn caused a significant decrease in cis-11-hexadecenal (Z11-16: Ald) production, female capability to attract males, and mating frequency. Further study demonstrated that sugar feeding (5% sugar) increased the transcription and enzyme activity of HaPYK, thereby facilitating sex pheromone biosynthesis. Moreover, pheromone biosynthesis activating neuropeptide (PBAN) upregulated HaPYK activity through protein kinase C (PKC), as shown by PKC-specific inhibitor analysis. Altogether, our results revealed that PBAN activated HaPYK by Ca(2+)/PKC, thereby regulating the synthesis of pyruvate and subsequent acetyl-CoA, ensuring the supply of sex pheromone precursor, and finally facilitating sex pheromone biosynthesis and mating behavior"
Keywords:		HaPYK Pkc Z11-16: Ald acetyl-CoA mating behavior sugar feeding;
Notes:		"PubMed-not-MEDLINEYao, Shuangyan Zhang, Yunhui Chang, Yanpeng Li, Xiang Zhao, Wenli An, Shiheng eng Switzerland 2021/08/24 Front Physiol. 2021 Aug 4; 12:707389. doi: 10.3389/fphys.2021.707389. eCollection 2021"