| Title: | What Role Might Non-Mating Receptors Play in Schizophyllum commune? |
| Author(s): | Wirth S; Freihorst D; Krause K; Kothe E; |
| Address: | "Friedrich Schiller University Jena, Institute of Microbiology, Microbial Communication, 25 07743 Neugasse Jena, Germany" |
| ISSN/ISBN: | 2309-608X (Electronic) 2309-608X (Linking) |
| Abstract: | "The B mating-type locus of the tetrapolar basidiomycete Schizophyllum commune encodes pheromones and pheromone receptors in multiple allelic specificities. This work adds substantial new evidence into the organization of the B mating-type loci of distantly related S. commune strains showing a high level of synteny in gene order and neighboring genes. Four pheromone receptor-like genes were found in the genome of S. commune with brl1, brl2 and brl3 located at the B mating-type locus, whereas brl4 is located separately. Expression analysis of brl genes in different developmental stages indicates a function in filamentous growth and mating. Based on the extensive sequence analysis and functional characterization of brl-overexpression mutants, a function of Brl1 in mating is proposed, while Brl3, Brl4 and Brl2 (to a lower extent) have a role in vegetative growth, possible determination of growth direction. The brl3 and brl4 overexpression mutants had a dikaryon-like, irregular and feathery phenotype, and they avoided the formation of same-clone colonies on solid medium, which points towards enhanced detection of self-signals. These data are supported by localization of Brl fusion proteins in tips, at septa and in not-yet-fused clamps of a dikaryon, confirming their importance for growth and development in S. commune" |
| Keywords: | B mating-type locus Schizophyllum commune mating pheromone receptor-like genes self-recognition; |
| Notes: | "PubMed-not-MEDLINEWirth, Sophia Freihorst, Daniela Krause, Katrin Kothe, Erika eng Graduate School 214/German Excellence Initiative/ Switzerland 2021/06/03 J Fungi (Basel). 2021 May 20; 7(5):399. doi: 10.3390/jof7050399" |
