| Title: | Two carboxylesterase genes in Plutella xylostella associated with sex pheromones and plant volatiles degradation |
| Author(s): | Wang MM; Long GJ; Guo H; Liu XZ; Wang H; Dewer Y; Li ZQ; Liu K; Zhang QL; Ma YF; He P; He M; |
| Address: | "State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, P. R. China. Bioassay Research Department, Central Agricultural Pesticide Laboratory, Agricultural Research Center, Dokki, Giza, Egypt. Key Laboratory of Tea Biology and Resource Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou, P. R. China" |
| ISSN/ISBN: | 1526-4998 (Electronic) 1526-498X (Linking) |
| Abstract: | "BACKGROUND: Carboxyl/cholinesterases (CCEs) are thought to play a pivotal role in the degradation of sex pheromones and plant-derived odorants in insects, but their exact biochemistry and physiological functions remain unclear. RESULTS: In this study, two paralogous antennae-enriched CCEs from Plutella xylostella (PxylCCE16a and 16c) were identified and functionally characterized. High-purity protein preparations of active recombinant PxylCCE16a and 16c have been obtained from Sf9 insect cells by Ni(2+) affinity purification. Our results revealed that the purified recombinant PxylCCE016c is able to degrade two sex pheromone components Z9-14:Ac and Z11-16:Ac at 27.64 +/- 0.79% and 24.40 +/- 3.07%, respectively, while PxylCCE016a presented relatively lower activity. Additionally, a similar difference in activity was measured in plant-derived odorants. Furthermore, both CCEs displayed obvious preferences for the two sex pheromone components, especially on Z11-16:Ac (K(m) values are in the range 7.82-45.06 mumol L(-1) ) which much lower than plant odorants (K(m) values are in the range 1290-4030 mumol L(-1) ). Furthermore, the activity of the two newly identified CCEs is pH-dependent. The activity at pH 6.5 is obviously higher than that at pH 5.0. Interestingly, only PxylCCE016c can be inhibited by a common esterase inhibitor triphenyl phosphate (TPP) with LC(50) of 1570 +/- 520 mumol L(-1) . CONCLUSION: PxylCCE16c plays a more essential role in odorant degradation than PxylCCE16a. Moreover, the current study provides novel potential pesticide targets for the notorious moth Plutella xylostella. (c) 2021 Society of Chemical Industry" |
| Keywords: | Animals Carboxylesterase/genetics Insect Proteins/genetics *Moths/genetics Odorants Pheromones Plants *Sex Attractants/pharmacology Plutella xylostella carboxylesterase kinetic parameter odorant degrading enzyme sex pheromone; |
| Notes: | "MedlineWang, Mei-Mei Long, Gui-Jun Guo, Huan Liu, Xuan-Zheng Wang, Hong Dewer, Youssef Li, Zhao-Qun Liu, Kun Zhang, Qiu-Liang Ma, Yun-Feng He, Peng He, Ming eng 31401750/National Natural Science Foundation of China/ 31860617/National Natural Science Foundation of China/ QKH-J [2019]1109/Natural Science Foundation of Guizhou Province of China/ QKH-J [2020]1Y077/Natural Science Foundation of Guizhou Province of China/ [2019]05/Program of talent cultivation of Guizhou University/ QKH[2017]2956/Science and Technology Support of Guizhou province/ 111 Program/Program of Introducing Talents of Discipline to Universities of China/ D20023/Program of Introducing Talents of Discipline to Universities of China/ 2017-33/Scientific Research Foundation of Guizhou University of China/ Qianjiaohe KY number (2020) 004/Frontiers Science Center for Asymmetric Synthesis and Medicinal Molecules, Department of Education, Guizhou Province/ England 2021/02/03 Pest Manag Sci. 2021 Jun; 77(6):2737-2746. doi: 10.1002/ps.6302. Epub 2021 Feb 22" |
