| Title: | Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites |
| Author(s): | Taware R; Taunk K; Pereira JAM; Shirolkar A; Soneji D; Camara JS; Nagarajaram HA; Rapole S; |
| Address: | "Proteomics Lab, National Centre for Cell Science (NCCS), Ganeshkhind, SPPU Campus, Pune, 411007, India. CQM - Centro de Quimica da Madeira, Universidade da Madeira, Campus Universitario da Penteada, Funchal, 9000-390, Portugal. Malignant Disease Treatment Centre, Military Hospital (Cardio Thoracic Centre), Armed Forces Medical College, Pune, 411040, India. Faculdade de Ciencias Exatas e da Engenharia, Universidade da Madeira, Campus Universitario da Penteada, Funchal, 9000-390, Portugal. Laboratory of Computational Biology, Centre for DNA fingerprinting & Diagnostics (CDFD), Nampally, Hyderabad, 500001, India. Department of Biotechnology & Bioinformatics, School of Life Sciences, University of Hyderabad, Hyderabad, 500046, India. Proteomics Lab, National Centre for Cell Science (NCCS), Ganeshkhind, SPPU Campus, Pune, 411007, India. rsrikanth@nccs.res.in" |
| DOI: | 10.1038/s41598-018-35854-x |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Head and neck cancer (HNC) is a heterogeneous malignant disease with distinct global distribution. Metabolic adaptations of HNC are significantly gaining clinical interests nowadays. Here, we investigated effects of HNC on differential expression of volatile metabolites in human saliva. We applied headspace solid phase microextraction coupled with gas chromatography-mass spectrometry analysis of saliva samples collected from 59 human subjects (HNC - 32, Control - 27). We identified and quantified 48 volatile organic metabolites (VOMs) and observed profound effects of HNC on these metabolites. These effects were VOM specific and significantly differed in the biologically comparable healthy controls. HNC induced changes in salivary VOM composition were well attributed to in vivo metabolic effects. A panel of 15 VOMs with variable importance in projection (VIP) score >1, false discovery rate (FDR) corrected p-value < 0.05 and log(2) fold change (log(2) FC) value of >/=0.58/ |
| Keywords: | "Adult Aged Aged, 80 and over Case-Control Studies Female Gas Chromatography-Mass Spectrometry/methods Head and Neck Neoplasms/*metabolism Humans Male Metabolic Networks and Pathways/*physiology Middle Aged ROC Curve Saliva/*metabolism Solid Phase Microext;" |
| Notes: | "MedlineTaware, Ravindra Taunk, Khushman Pereira, Jorge A M Shirolkar, Amey Soneji, Dharmesh Camara, Jose S Nagarajaram, H A Rapole, Srikanth eng BT/IN/NEW INDIGO/03/RS/2013/Department of Biotechnology, Ministry of Science and Technology (DBT)/International DST/IMRCD/EU/Inno-Indigo/NCDs-CAPomics/2015/Department of Science and Technology, Ministry of Science and Technology (DST)/International Research Support, Non-U.S. Gov't England 2018/12/12 Sci Rep. 2018 Dec 7; 8(1):17725. doi: 10.1038/s41598-018-35854-x" |
