« Previous Abstract"Synthetic and Natural Insecticides: Gas, Liquid, Gel and Solid Formulations for Stored-Product and Food-Industry Pest Control"    Next Abstract"Presence of long-lasting peripheral adaptation in oblique-banded leafroller, Choristoneura rosaceana and absence of such adaptation in redbanded leafroller, Argyrotaenia velutinana" »

J Vis Exp

Title:		Vertical T-maze choice assay for arthropod response to odorants
Author(s):		Stelinski L; Tiwari S;
Address:		"Entomology and Nematology Department, University of Florida, USA. stelinski@ufl.edu"
Journal Title:		J Vis Exp
Year:		2013
Volume:		20130214
Issue:		72
Page Number:		-
DOI:		10.3791/50229
ISSN/ISBN:		1940-087X (Electronic) 1940-087X (Linking)
Abstract:		"Given the economic importance of insects and arachnids as pests of agricultural crops, urban environments or as vectors of plant and human diseases, various technologies are being developed as control tools. A subset of these tools focuses on modifying the behavior of arthropods by attraction or repulsion. Therefore, arthropods are often the focus of behavioral investigations. Various tools have been developed to measure arthropod behavior, including wind tunnels, flight mills, servospheres, and various types of olfactometers. The purpose of these tools is to measure insect or arachnid response to visual or more often olfactory cues. The vertical T-maze olfactometer described here measures choices performed by insects in response to attractants or repellents. It is a high throughput assay device that takes advantage of the positive phototaxis (attraction to light) and negative geotaxis (tendency to walk or fly upward) exhibited by many arthropods. The olfactometer consists of a 30 cm glass tube that is divided in half with a Teflon strip forming a T-maze. Each half serves as an arm of the olfactometer enabling the test subjects to make a choice between two potential odor fields in assays involving attractants. In assays involving repellents, lack of normal response to known attractants can also be measured as a third variable"
Keywords:		"Animals Arthropods/*physiology Behavior, Animal/physiology Female Insect Repellents Male Maze Learning *Odorants Olfactory Perception/physiology Pest Control, Biological/*methods *Pesticides Pheromones;"
Notes:		"MedlineStelinski, Lukasz Tiwari, Siddharth eng Research Support, Non-U.S. Gov't Video-Audio Media 2013/02/27 J Vis Exp. 2013 Feb 14; (72):50229. doi: 10.3791/50229"