| Title: | Cellular scent of influenza virus infection |
| Author(s): | Aksenov AA; Sandrock CE; Zhao W; Sankaran S; Schivo M; Harper R; Cardona CJ; Xing Z; Davis CE; |
| Address: | "Department of Mechanical and Aeronautical Engineering, University of California, Davis, One Shields Ave, Davis, CA 95616 (USA)" |
| ISSN/ISBN: | 1439-7633 (Electronic) 1439-4227 (Print) 1439-4227 (Linking) |
| Abstract: | "Volatile organic compounds (VOCs) emanating from humans have the potential to revolutionize non-invasive diagnostics. Yet, little is known about how these compounds are generated by complex biological systems, and even less is known about how these compounds are reflective of a particular physiological state. In this proof-of-concept study, we examined VOCs produced directly at the cellular level from B lymphoblastoid cells upon infection with three live influenza virus subtypes: H9N2 (avian), H6N2 (avian), and H1N1 (human). Using a single cell line helped to alleviate some of the complexity and variability when studying VOC production by an entire organism, and it allowed us to discern marked differences in VOC production upon infection of the cells. The patterns of VOCs produced in response to infection were unique for each virus subtype, while several other non-specific VOCs were produced after infections with all three strains. Also, there was a specific time course of VOC release post infection. Among emitted VOCs, production of esters and other oxygenated compounds was particularly notable, and these may be attributed to increased oxidative stress resulting from infection. Elucidating VOC signatures that result from the host cells response to infection may yield an avenue for non-invasive diagnostics and therapy of influenza and other viral infections" |
| Keywords: | "B-Lymphocytes/cytology/*metabolism/virology Biomarkers/metabolism Cell Line Gas Chromatography-Mass Spectrometry Humans Influenza A Virus, H1N1 Subtype/*metabolism Influenza A Virus, H9N2 Subtype/*metabolism Influenza, Human/metabolism/pathology/*virology;" |
| Notes: | "MedlineAksenov, Alexander A Sandrock, Christian E Zhao, Weixiang Sankaran, Shankar Schivo, Michael Harper, Richart Cardona, Carol J Xing, Zheng Davis, Cristina E eng T32 ES007059/ES/NIEHS NIH HHS/ 8KL2TR000134-07K12/TR/NCATS NIH HHS/ T35 ES007301/ES/NIEHS NIH HHS/ UL1 TR000002/TR/NCATS NIH HHS/ T32-ES007059/ES/NIEHS NIH HHS/ T32-HL007013/HL/NHLBI NIH HHS/ T32 HL007013/HL/NHLBI NIH HHS/ KL2 TR000134/TR/NCATS NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Germany 2014/04/11 Chembiochem. 2014 May 5; 15(7):1040-8. doi: 10.1002/cbic.201300695. Epub 2014 Apr 9" |
