| Title: | Quantitative analysis of volatile metabolites released in vitro by bacteria of the genus Stenotrophomonas for identification of breath biomarkers of respiratory infection in cystic fibrosis |
| Author(s): | Shestivska V; Dryahina K; Nunvar J; Sovova K; Elhottova D; Nemec A; Smith D; Spanel P; |
| Address: | "J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 3, 18223 Prague 8, Czech Republic" |
| DOI: | 10.1088/1752-7155/9/2/027104 |
| ISSN/ISBN: | 1752-7163 (Electronic) 1752-7155 (Linking) |
| Abstract: | "The aim of the present study was to characterize the volatile metabolites produced by genotypically diverse strains of the Stenotrophomonas genus in order to evaluate their potential as biomarkers of lung infection by non-invasive breath analysis. Volatile organic compounds (VOCs) emitted from 15 clinical and five environmental strains belonging to different genogroups of Stenotrophomonas maltophilia (n = 18) and Stenotrophomonas rhizophila (n = 2) cultured in Mueller-Hinton Broth (MHB) liquid media were analysed by gas chromatography mass spectrometry (GC-MS) and selected ion flow tube mass spectrometry (SIFT-MS). Several VOCs were detected in high concentration, including ammonia, propanol, dimethyl disulphide propanol and dimethyl disulphide. The GC-MS measurements showed that all 15 clinical strains produced similar headspace VOCs compositions, and SIFT-MS quantification showed that the rates of production of the VOCs by the genotypically distinct strains were very similar. All in vitro cultures of both the Stenotrophomonas species were characterised by efficient production of two isomers of methyl butanol, which can be described by known biochemical pathways and which is absent in other pathogens, including Pseudomonas aeruginosa. These in-vitro data indicate that methyl butanol isomers may be exhaled breath biomarkers of S. maltophilia lung infection in patients with cystic fibrosis" |
| Keywords: | 1-Propanol/analysis/metabolism Ammonia/analysis/metabolism Biomarkers/analysis Breath Tests/methods Cystic Fibrosis/*complications Disulfides/analysis/metabolism Gas Chromatography-Mass Spectrometry Genotype Gram-Negative Bacterial Infections/complication; |
| Notes: | "MedlineShestivska, Violetta Dryahina, Kseniya Nunvar, Jaroslav Sovova, Kristyna Elhottova, Dana Nemec, Alexandr Smith, David Spanel, Patrik eng England 2015/04/02 J Breath Res. 2015 Apr 1; 9(2):027104. doi: 10.1088/1752-7155/9/2/027104" |
