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Yeast

Title:		"The yeast community of Conotelus sp. (Coleoptera: Nitidulidae) in Brazilian passionfruit flowers (Passiflora edulis) and description of Metschnikowia amazonensis sp. nov., a large-spored clade yeast"
Author(s):		Santos ARO; Lee DK; Ferreira AG; do Carmo MC; Rondelli VM; Barros KO; Hsiang T; Rosa CA; Lachance MA;
Address:		"Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. Department of Biology, University of Western Ontario, London, Ontario, Canada. Departamento de Agronomia, Universidade Federal de Rondonia, Rolim de Moura, Rondonia, Brazil. Departamento de Agronomia e Entomologia, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil. School of Environmental Sciences, University of Guelph, Guelph, Ontario, Canada"
Journal Title:		Yeast
Year:		2020
Volume:		20200203
Issue:		3
Page Number:		253 - 260
DOI:		10.1002/yea.3453
ISSN/ISBN:		1097-0061 (Electronic) 0749-503X (Linking)
Abstract:		"Species of the nitidulid beetle Conotelus found in flowers of Convolvulaceae and other plants across the New World and in Hawaii consistently harbour a yeast community dominated by one or more large-spored Metschnikowia species. We investigated the yeasts found in beetles and flowers of cultivated passionfruit in Rondonia state, in the Amazon biome of Brazil, where a Conotelus species damages the flowers and hinders fruit production. A sample of 46 beetles and 49 flowers yielded 86 and 83 yeast isolates, respectively. Whereas the flower community was dominated by Kodamaea ohmeri and Kurtzmaniella quercitrusa, the major yeasts recovered from beetles were Wickerhamiella occidentalis, which is commonly isolated from this community, and a novel species of large-spored Metschnikowia in the arizonensis subclade, which we describe here as Metschnikowia amazonensis sp. nov. Phylogenetic analyses based on barcode sequences (ITS-D1/D2) and a multigene alignment of 11,917 positions (genes ura2, msh6, and pmt2) agreed to place the new species as a sister to Metschnikowia arizonensis, a rare species known only from one locality in Arizona. The two form sterile asci when mated, which is typical of related members of the clade. The alpha pheromone of the new species is unique but typical of the subclade. The type of M. amazonensis sp. nov. is UFMG-CM-Y6309(T) (ex-type CBS 16156(T) , mating type a), and the designated allotype (mating type alpha) is UFMG-CM-Y6307(A) (CBS 16155(A) ). MycoBank MB 833560"
Keywords:		"Animals Brazil Coleoptera/*microbiology/parasitology DNA, Fungal/analysis Flowers/*microbiology/parasitology Metschnikowia/*classification/genetics/isolation & purification/physiology Microbiota/*physiology Mycological Typing Techniques Passiflora/*microb;"
Notes:		"MedlineSantos, Ana Raquel de Oliveira Lee, Dong Kyung Ferreira, Andressa Graebin do Carmo, Marina Conceicao Rondelli, Vando Miossi Barros, Katharina O Hsiang, Tom Rosa, Carlos A Lachance, Marc-Andre eng Research Support, Non-U.S. Gov't England 2020/02/06 Yeast. 2020 Mar; 37(3):253-260. doi: 10.1002/yea.3453. Epub 2020 Feb 3"