| Title: | Unique microbial-derived volatile organic compounds in portal venous circulation in murine non-alcoholic fatty liver disease |
| Author(s): | Reid DT; McDonald B; Khalid T; Vo T; Schenck LP; Surette MG; Beck PL; Reimer RA; Probert CS; Rioux KP; Eksteen B; |
| Address: | "Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, Canada. Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, Canada; Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada. Department of Gastroenterology, Institute of Translational Medicine, University of Liverpool, Liverpool, UK. Department of Medicine, Department of Biochemistry and Biomedical Sciences, Farncombe Family Digestive Health Research Institute, Faculty of Health Sciences, McMaster University, Hamilton, ON, Canada. Department of Biochemistry & Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, Canada; Faculty of Kinesiology, University of Calgary, Calgary, Canada. Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, Canada; Department of Medicine, Division of Gastroenterology and Hepatology, Department of Microbiology and Infectious Diseases, Gastrointestinal Research Group, University of Calgary, Calgary, Canada. Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, Canada. Electronic address: b.eksteen@ucalgary.ca" |
| DOI: | 10.1016/j.bbadis.2016.04.005 |
| ISSN/ISBN: | 0006-3002 (Print) 0006-3002 (Linking) |
| Abstract: | "BACKGROUND AND AIMS: Non-alcoholic fatty liver disease is now the leading liver disease in North America. The progression of non-alcoholic fatty liver disease to the inflammatory condition, non-alcoholic steatohepatitis is complex and currently not well understood. Intestinal microbial dysbiosis has been implicated in the development of non-alcoholic fatty liver disease and progression of non-alcoholic steatohepatitis. Volatile organic compounds are byproducts of microbial metabolism in the gut that may enter portal circulation and have hepatotoxic effects contributing to the pathogenesis of non-alcoholic steatohepatitis. To test this hypothesis, we measured volatile organic compounds in cecal luminal contents and portal venous blood in a mouse model of non-alcoholic steatohepatitis. METHODS: Gas chromatography-mass spectrometry analysis was conducted on cecal content and portal vein blood for volatile organic compound detection from mice fed a methionine and choline deficient diet, which induces non-alcoholic steatohepatitis. The colonic microbiome was studied by 16S rRNA gene amplification using the Illumina MiSeq platform. RESULTS: Sixty-eight volatile organic compounds were detected in cecal luminal content, a subset of which was also present in portal venous blood. Importantly, differences in portal venous volatile organic compounds were associated with diet-induced steatohepatitis establishing a biochemical link between gut microbiota-derived volatile organic compounds and increased susceptibility to non-alcoholic steatohepatitis. CONCLUSION: Our model creates a novel tool to further study the role of gut-derived volatile organic compounds in the pathogenesis of non-alcoholic steatohepatitis" |
| Keywords: | "Animals Bacteria/isolation & purification Cells, Cultured Disease Models, Animal Inflammation/*microbiology/pathology Inflammation Mediators/analysis Liver/*blood supply/microbiology/pathology Macrophages/microbiology/pathology Male Mice, Inbred C57BL Mic;" |
| Notes: | "MedlineReid, D T McDonald, B Khalid, T Vo, T Schenck, L P Surette, M G Beck, P L Reimer, R A Probert, C S Rioux, K P Eksteen, B eng Research Support, Non-U.S. Gov't Netherlands 2016/04/17 Biochim Biophys Acta. 2016 Jul; 1862(7):1337-44. doi: 10.1016/j.bbadis.2016.04.005. Epub 2016 Apr 13" |
