| Title: | "Two-hybrid cloning and characterization of OSH3, a yeast oxysterol-binding protein homolog" |
| Address: | "Department of Microbiology, College of Natural Sciences, Chungnam National University, Taejeon 305-764, Republic of Korea" |
| Journal Title: | Biochem Biophys Res Commun |
| DOI: | 10.1016/S0006-291X(02)00288-7 |
| ISSN/ISBN: | 0006-291X (Print) 0006-291X (Linking) |
| Abstract: | "We identify Osh3p, one of seven yeast oxysterol-binding protein (OSBP) homologs, by its protein-protein interactions with a DEAD-box RNA helicase, Rok1p. The ROK1 gene was initially identified by its ability on a high-copy number plasmid to suppress the nuclear fusion defect caused by the kem1 null mutation. Our results show that OSH3 also affects nuclear fusion in a kem1-specific manner; the nuclear fusion defect of kem1 was intensified by the multicopy expression of OSH3. The Osh3p synthesis was highly induced by alpha-mating pheromone. We also found that OSH3 overexpression promoted filamentation growth of the Sigma1278b wild-type strain and suppressed the filamentation growth defect of the ste12 mutation. These results lead us to a new understanding of cellular functions of the yeast OSBPs" |
| Keywords: | "Carrier Proteins/*genetics/*physiology Cell Nucleus/metabolism Cloning, Molecular DEAD-box RNA Helicases Mating Factor Membrane Fusion Mutation Peptides/pharmacology RNA Helicases/metabolism RNA, Fungal/biosynthesis Saccharomyces cerevisiae/genetics/growt;" |
| Notes: | "MedlinePark, Young-Un Hwang, Oksun Kim, Jinmi eng Research Support, Non-U.S. Gov't 2002/06/11 Biochem Biophys Res Commun. 2002 May 3; 293(2):733-40. doi: 10.1016/S0006-291X(02)00288-7" |
