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J Chem Ecol

Title:		Phosphorylation of native porcine olfactory binding proteins
Author(s):		Nagnan-Le Meillour P; Le Danvic C; Brimau F; Chemineau P; Michalski JC;
Address:		"INRA, UMR 8576 CNRS/Universite Lille1, Unite de Glycobiologie Structurale et Fonctionnelle, F-59655, Villeneuve d'Ascq, Cedex, France. patricia.le-meillour@univ-lille1.fr"
Journal Title:		J Chem Ecol
Year:		2009
Volume:		20090707
Issue:		7
Page Number:		752 - 760
DOI:		10.1007/s10886-009-9663-z
ISSN/ISBN:		1573-1561 (Electronic) 0098-0331 (Linking)
Abstract:		"The identification of various isoforms of olfactory binding proteins is of major importance to elucidate their involvement in detection of pheromones and other odors. Here, we report the characterization of the phosphorylation of OBP (odorant binding protein) and Von Ebner's gland protein (VEG) from the pig, Sus scrofa. After labeling with specific antibodies raised against the three types of phosphorylation (Ser, Thr, Tyr), the phosphate-modified residues were mapped by using the beta-elimination followed by Michael addition of dithiothreitol (BEMAD) method. Eleven phosphorylation sites were localized in the pOBP sequence and nine sites in the VEG sequence. OBPs are secreted by Bowman's gland cells in the extracellular mucus lining the nasal cavity. After tracking the secretion pathway in the rough endoplasmic reticulum of these cells, we hypothesize that these proteins may be phosphorylated by ectokinases that remain to be characterized. The existence of such a regulatory mechanism theoretically increases the number of OBP variants, and it suggests a more specific role for OBPs in odorant coding than the one of odorant solubilizer and transporter"
Keywords:		"Animals Gas Chromatography-Mass Spectrometry Lipocalin 1/*chemistry/metabolism Olfactory Mucosa/chemistry Peptide Mapping Phosphorylation Receptors, Odorant/*chemistry/metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sus scrofa;"
Notes:		"MedlineNagnan-Le Meillour, Patricia Le Danvic, Chrystelle Brimau, Fanny Chemineau, Philippe Michalski, Jean-Claude eng 2009/07/07 J Chem Ecol. 2009 Jul; 35(7):752-60. doi: 10.1007/s10886-009-9663-z. Epub 2009 Jul 7"