| Title: | Yeast KEX2 genes encodes an endopeptidase homologous to subtilisin-like serine proteases |
| Author(s): | Mizuno K; Nakamura T; Ohshima T; Tanaka S; Matsuo H; |
| Address: | "Department of Biochemistry, Miyazaki Medical College, Japan" |
| Journal Title: | Biochem Biophys Res Commun |
| DOI: | 10.1016/s0006-291x(88)80832-5 |
| ISSN/ISBN: | 0006-291X (Print) 0006-291X (Linking) |
| Abstract: | "Yeast Saccharomyces cerevisiae KEX2 gene previously isolated, was characterized as the gene encoding a calcium-dependent endopeptidase required for processing of precursors of alpha-factor and killer toxin. In this study, we report the amino acid sequence of the KEX2 gene product deduced from nucleotide sequencing. Our results indicate that the KEX2 gene contains a 2,442-bp open reading frame encoding a polypeptide of 814 amino acids. The deduced amino acid sequence contains a region extensively homologous to the members of subtilisin-like serine protease family near the N-terminus. A putative membrane-spanning domain near the C-terminus was also detected. These facts indicate that the KEX2-encoded protein may function as a membrane-bound, subtilisin-like serine protease" |
| Keywords: | "Amino Acid Sequence Base Sequence Cloning, Molecular DNA Restriction Enzymes Endopeptidases/*genetics Escherichia coli/genetics *Genes *Genes, Fungal Mating Factor Molecular Sequence Data Mutation Nucleotide Mapping Peptides/genetics Saccharomyces cerevis;" |
| Notes: | "MedlineMizuno, K Nakamura, T Ohshima, T Tanaka, S Matsuo, H eng 1988/10/14 Biochem Biophys Res Commun. 1988 Oct 14; 156(1):246-54. doi: 10.1016/s0006-291x(88)80832-5" |
