| Title: | Plant-Insect Bioassay for Testing Arabidopsis Resistance to the Generalist Herbivore Spodoptera littoralis |
| Address: | "Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry, Halle (Saale), Germany. Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry, Halle (Saale), Germany. debora.gasperini@ipb-halle.de" |
| DOI: | 10.1007/978-1-0716-0142-6_5 |
| ISSN/ISBN: | 1940-6029 (Electronic) 1064-3745 (Linking) |
| Abstract: | "Jasmonates are essential engineers of plant defense responses against many pests, including herbivorous insects. Herbivory induces the production of jasmonic acid (JA) and its bioactive conjugate jasmonoyl-L-isoleucine (JA-Ile), which then triggers a large transcriptional reprogramming to promote plant acclimation. The contribution of the JA pathway, including its components and regulators, to defense responses against insect herbivory can be evaluated by conducting bioassays with a wide range of host plants and insect pests. Here, we describe a detailed and reproducible protocol for testing feeding behavior of the generalist herbivore Spodoptera littoralis on the model plant Arabidopsis thaliana and hence infer the contribution of JA-mediated plant defense responses to a chewing insect" |
| Keywords: | Animals Arabidopsis/*parasitology *Biological Assay Cyclopentanes/metabolism *Disease Resistance *Herbivory *Host-Parasite Interactions Oxylipins/metabolism Phenotype Spodoptera/*physiology Arabidopsis thaliana Caterpillar Generalist herbivore Jasmonic ac; |
| Notes: | "MedlineMielke, Stefan Gasperini, Debora eng Research Support, Non-U.S. Gov't 2019/11/18 Methods Mol Biol. 2020; 2085:69-78. doi: 10.1007/978-1-0716-0142-6_5" |
