| Title: | ras1 and pat1 alleles interact to quantitatively and qualitatively alter conjugation in fission yeast |
| Author(s): | Mach KE; Cheng Q; Albright CF; |
| Address: | "655 Light Hall, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, USA" |
| ISSN/ISBN: | 0172-8083 (Print) 0172-8083 (Linking) |
| Abstract: | "To identify novel components of ras1+ signalling in Schizosaccharomyces pombe, extragenic suppressors of the mating defect of ras1 effector mutants were isolated. A novel allele of pat1, pat1-e1, was isolated that increases the mating of ras1-D43E mutants to near wild-type levels but does not suppress the mating defect of ras1-I41M, ras1-Y37F, or ras1-Y45I mutants. This allele-specific suppression is not a characteristic of all pat1 alleles since pat1-3 and pat1-114 partially and equally suppress ras1-D43E and ras1-I41M mutants. Analysis of mating cultures showed that ras1-D43E and pat1-e1 interact to qualitatively alter the mating response. While pat1-e1 ras1-D43E cells were delayed in agglutination, cell-cycle delay, and mat1-Pm transcription, they induce mat1-Mc at the same time and mate more rapidly than other mating cultures. These results suggest that pheromone signalling, but not nutritional signalling, is delayed in pat1-e1 ras1-D43E cells. We hypothesize that this delay causes an elevated pheromone response and thus suppression of the mating defect of the ras1-D43E mutant by pat1-e1" |
| Keywords: | "Alleles Cell Division/genetics Conjugation, Genetic/*genetics DNA-Binding Proteins/*genetics Fluorescent Dyes/metabolism *Fungal Proteins Gene Expression Regulation, Fungal/genetics Genes, Fungal/genetics Indoles/metabolism Microscopy, Fluorescence Pherom;" |
| Notes: | "MedlineMach, K E Cheng, Q Albright, C F eng GM-51952/GM/NIGMS NIH HHS/ T32-CA09582/CA/NCI NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1998/09/24 Curr Genet. 1998 Sep; 34(3):172-82. doi: 10.1007/s002940050383" |
