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Mol Gen Genet

Title:		"Only one of the paired Schizophyllum commune A alpha mating-type, putative homeobox genes encodes a homeodomain essential for A alpha-regulated development"
Author(s):		Luo Y; Ullrich RC; Novotny CP;
Address:		"Department of Microbiology and Molecular Genetics, University of Vermont, Burlington 05405"
Journal Title:		Mol Gen Genet
Year:		1994
Volume:		244
Issue:		3
Page Number:		318 - 324
DOI:		10.1007/BF00285460
ISSN/ISBN:		0026-8925 (Print) 0026-8925 (Linking)
Abstract:		"The A alpha mating-type locus is one of four master regulatory loci controlling sexual development in Schizophyllum commune. The A alpha locus contains two homeobox genes, Y and Z, encoding two homeodomain-related proteins, Y and Z. Y and Z are each multiallelic genes. When haploid strains form fusion cells, only particular combinations of Y and Z alleles activate A alpha-regulated sexual development. The role of the putative homeodomain was examined in several Y and Z alleles by site-directed mutagenesis of regions critical to secondary structure and function of homeodomains. Mutations of the Z homeobox do not affect the function of Z proteins in A alpha-activated development, but mutations of Y homeoboxes destroy the ability of Y proteins to activate development. We conclude that only one of two A alpha homeodomain-related regulators relies upon the homeodomain motif to effect gene expression in sexual development. This conclusion affords a refinement of our working hypothesis for the mechanism by which A alpha proteins may regulate target gene expression. On the basis of our results with the Z protein, we speculate that the DNA-binding motifs of some transcriptional regulators may be lost or modified during evolution once these regulators have been recruited to participate in complexes with other DNA-binding proteins"
Keywords:		"Amino Acid Sequence DNA Mutational Analysis Fungal Proteins/genetics *Gene Expression Regulation, Fungal Genes, Fungal/*genetics Genes, Homeobox/*genetics *Genes, Mating Type, Fungal Mating Factor Models, Genetic Molecular Sequence Data Morphogenesis/gene;"
Notes:		"MedlineLuo, Y Ullrich, R C Novotny, C P eng GM 34023/GM/NIGMS NIH HHS/ Comparative Study Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Germany 1994/08/02 Mol Gen Genet. 1994 Aug 2; 244(3):318-24. doi: 10.1007/BF00285460"