| Title: | Searching for Potential Markers of Glomerulopathy in Urine by HS-SPME-GCxGC TOFMS |
| Author(s): | Ligor T; Zawadzka J; Straczynski G; Gonzalez Paredes RM; Wenda-Piesik A; Ratiu IA; Muszytowski M; |
| Address: | "Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 87-100 Torun, Poland. Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, 87-100 Torun, Poland. Department of Nephrology, Diabetology and Internal Medicine, Nicolaus Copernicus University, Rydygier Hospital, 87-100 Torun, Poland. USL Ltd., 43-110 Tychy, Poland. Department of Analytical Chemistry, Nutrition and Food Sciences, University of Salamanca, 37008 Salamanca, Spain. Department of Plant Growth Principles and Experimental Methods, UTP University of Science and Technology, 85-796 Bydgoszcz, Poland. 'Raluca Ripan' Institute for Research in Chemistry, Babes-Bolyai University, 30 Fantanele, RO-400239 Cluj Napoca, Romania" |
| DOI: | 10.3390/molecules26071817 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Volatile organic compounds (VOCs) exiting in urine are potential biomarkers of chronic kidney diseases. Headspace solid phase microextraction (HS-SPME) was applied for extraction VOCs over the urine samples. Volatile metabolites were separated and identified by means of two-dimensional gas chromatography and time of flight mass spectrometry (GC x GC TOF MS). Patients with glomerular diseases (n = 27) and healthy controls (n = 20) were recruited in the study. Different VOCs profiles were obtained from patients and control. Developed methodology offers the opportunity to examine the metabolic profile associated with glomerulopathy. Four compounds found in elevated amounts in the patients group, i.e., methyl hexadecanoate; 9-hexadecen-1-ol; 6,10-dimethyl-5,9-undecadien-2-one and 2-pentanone were proposed as markers of glomerular diseases" |
| Keywords: | "Biomarkers/urine Female *Gas Chromatography-Mass Spectrometry Humans Male Middle Aged Renal Insufficiency, Chronic/*urine Volatile Organic Compounds/*urine comprehensive two-dimensional gas chromatography kidney diseases urine analysis volatile organic co;" |
| Notes: | "MedlineLigor, Tomasz Zawadzka, Joanna Straczynski, Grzegorz Gonzalez Paredes, Rosa M Wenda-Piesik, Anna Ratiu, Ileana Andreea Muszytowski, Marek eng Clinical Trial Switzerland 2021/04/04 Molecules. 2021 Mar 24; 26(7):1817. doi: 10.3390/molecules26071817" |
