| Title: | Proteome analysis of Arabidopsis seedlings exposed to bacterial volatiles |
| Author(s): | Kwon YS; Ryu CM; Lee S; Park HB; Han KS; Lee JH; Lee K; Chung WS; Jeong MJ; Kim HK; Bae DW; |
| Address: | "Department of Applied Biology and Research Institute of Life Science, Gyeongsang National University, Jinju, 660-701, South Korea" |
| DOI: | 10.1007/s00425-010-1259-x |
| ISSN/ISBN: | 1432-2048 (Electronic) 0032-0935 (Linking) |
| Abstract: | "Plant root-associated bacteria (rhizobacteria) elicit plant basal immunity referred to as induced systemic resistance (ISR) against multiple pathogens. Among multi-bacterial determinants involving such ISR, the induction of ISR and promotion of growth by bacterial volatile compounds was previously reported. To exploit global de novo expression of plant proteins by bacterial volatiles, proteomic analysis was performed after exposure of Arabidopsis plants to the rhizobacterium Bacillus subtilis GB03. Ethylene biosynthesis enzymes were significantly up-regulated. Analysis by quantitative reverse transcriptase polymerase chain reaction confirmed that ethylene biosynthesis-related genes SAM-2, ACS4, ACS12, and ACO2 as well as ethylene response genes, ERF1, GST2, and CHIB were up-regulated by the exposure to bacterial volatiles. More interestingly, the emission of bacterial volatiles significantly up-regulated both key defense mechanisms mediated by jasmonic acid and salicylic acid signaling pathways. In addition, high accumulation of antioxidant proteins also provided evidence of decreased sensitivity to reactive oxygen species during the elicitation of ISR by bacterial volatiles. The present results suggest that the proteomic analysis of plant defense responses in bacterial volatile-mediated ISR can reveal the mechanisms of plant basal defenses orchestrated by endogenous ethylene production pathways and the generation of reactive oxygen species" |
| Keywords: | "Antioxidants/metabolism Arabidopsis/growth & development/*metabolism Arabidopsis Proteins/*metabolism Bacillus subtilis/*metabolism Base Sequence Chlorophyll/metabolism DNA Primers *Proteome Reverse Transcriptase Polymerase Chain Reaction Spectrometry, Ma;" |
| Notes: | "MedlineKwon, Young Sang Ryu, Choong-Min Lee, Soohyun Park, Hyo Bee Han, Ki Soo Lee, Jung Han Lee, Kyunghee Chung, Woo Sik Jeong, Mi-Jeong Kim, Hee Kyu Bae, Dong-Won eng Research Support, Non-U.S. Gov't Germany 2010/09/08 Planta. 2010 Nov; 232(6):1355-70. doi: 10.1007/s00425-010-1259-x. Epub 2010 Sep 4" |
