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Biochim Biophys Acta

Title:		Steroid profiling in the study of rat testicular steroidogenesis
Author(s):		Kwan TK; Pertiwi AK; Taylor NF; Gower DB;
Address:		"Department of Biochemistry, Faculty of Medicine, University of Malaya, Kuala Lumpur"
Journal Title:		Biochim Biophys Acta
Year:		1988
Volume:		962
Issue:		2
Page Number:		214 - 219
DOI:		10.1016/0005-2760(88)90162-2
ISSN/ISBN:		0006-3002 (Print) 0006-3002 (Linking)
Abstract:		"Twenty authentic steroids, derivatized as O-methyl oximes (MO), trimethylsilyl (TMS) ethers or as MO-TMS ethers have been subjected to capillary gas chromatography using two different columns. Virtually all of the steroid derivatives have been resolved, one difficult pair to separate being 5,16-androstadien-3 beta-ol and 5 alpha-androst-16-en-3 beta-ol on the non-selective phase OV-1. Where syn and anti forms of MO derivatives arose, these were also resolved under the conditions utilised. This technique of 'steroid profiling' has been applied to the separation and quantification of metabolites of pregnenolone which were formed during incubations of the microsomal and cytosolic fractions from rat testes. The majority of the metabolites were found in the microsomal incubation. These compounds included some odorous 16-androstenes as well as other C21 and C19 steroids, the formation of which was consistent with the 5-ene and 4-ene pathways of testosterone biosynthesis being operative. In addition, evidence was obtained for 16 alpha-hydroxylation of C21 steroids. Very much less metabolic activity was found in the cytosolic fraction of rat testes. Metabolic pathways have been proposed which both confirm and extend earlier work. We conclude that the rat testis can only form some of the odorous, possibly pheromonal, 16-androstenes and that these are quantitatively less important than in the porcine testis"
Keywords:		"Animals *Aryl Hydrocarbon Hydroxylases Biotransformation Chromatography, Gas Cytochrome P450 Family 2 Cytosol/analysis Gonadal Steroid Hormones/*analysis/metabolism/standards Male Microsomes/analysis Pregnenolone/metabolism Rats Rats, Inbred Strains Refer;"
Notes:		"MedlineKwan, T K Pertiwi, A K Taylor, N F Gower, D B eng Research Support, Non-U.S. Gov't Netherlands 1988/09/23 Biochim Biophys Acta. 1988 Sep 23; 962(2):214-9. doi: 10.1016/0005-2760(88)90162-2"