| Title: | Structure of a yeast pheromone gene (MF alpha): a putative alpha-factor precursor contains four tandem copies of mature alpha-factor |
| DOI: | 10.1016/0092-8674(82)90298-7 |
| ISSN/ISBN: | 0092-8674 (Print) 0092-8674 (Linking) |
| Abstract: | "We have cloned and sequenced a gene (MF alpha) coding for alpha-factor, a tridecapeptide mating factor secreted by yeast alpha cells. A plasmid carrying the MF alpha gene was identified by screening for production of alpha-factor by mat alpha 2 mutants, which fail to secrete alpha-factor because of simultaneous synthesis and degradation of the factor. The cloned segment codes for four mature alpha-factor within a putative precursor of 165 amino acids. The putative precursor begins as a signal sequence for secretion. The next segment, of approximately 60 amino acids, contains three potential glycosylation sites. The carboxy-terminal half of the precursor contains four tandem copies of mature alpha-factor, each preceded by spacer peptides of six or eight amino acids (variations of Lys-Arg-Glu-Ala-Asp-Ala-Glu-Ala), which are hypothesized to contain proteolytic processing signals" |
| Keywords: | "Amino Acid Sequence Animals Base Sequence Cloning, Molecular Fungal Proteins/*genetics Genes Pheromones/*genetics Protein Precursors/genetics Protein Processing, Post-Translational Saccharomyces cerevisiae/*genetics;" |
| Notes: | "MedlineKurjan, J Herskowitz, I eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1982/10/01 Cell. 1982 Oct; 30(3):933-43. doi: 10.1016/0092-8674(82)90298-7" |
