| Title: | Properties of mouse vomeronasal receptor and assessment of its role in pheromone signalling |
| Author(s): | Kannan S; Mareeswaran P; Krishnan M; Achiraman S; Spencer N; Nytingel BF; |
| Address: | "Proteomics & Molecular Cell Physiology Laboratory, Department of Zoology, Bharathiar University, Coimbatore-641 046, TN, India. sk_protein@buc.edu.in" |
| Journal Title: | Rapid Commun Mass Spectrom |
| ISSN/ISBN: | 1097-0231 (Electronic) 0951-4198 (Linking) |
| Abstract: | "Vomeronasal type 2 receptor (V2Rx) from Swiss mouse (Mus musculus (L.)) was analyzed by high-resolution ion-exchange chromatography, reversed-phase high-performance liquid chromatography (RP-HPLC), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS), Ion Spray tandem mass spectrometry (MS/MS), 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and 1-aminoanthracene (1-AMA) fluorometric assay. Vomeronasal sensory neuronal cell bound proteins were resolved into major protein peaks. Several proteins were identified and subsequently purified as the V2Rx receptor on 10% SDS-PAGE with trace amounts of other protein bands. The molecular weight of the identified V2Rx was 109 kDa. MALDI-TOF and micro-sequencing experiments demonstrated that the identified V2Rx receptor shared considerable sequence similarity with vomeronasal receptor type 2 (NCBI Accession Number AB267725), which is a seven transmembrane peptide with 912 amino acid residues. The molecular characterization revealed that the N-terminus of the V2Rx receptor contained the 11GAEAAE16 domain involved in pheromone signalling. The biometric assay (octanamine-V2Rx binding) showed the identified V2Rx receptor and mouse sex pheromone to 2-octanamine (methyl heptyl) in a 1:1 ratio. Uptake of odourants determined in physiological condition showed enhanced V2Rx receptors as volatile hydrophobic pheromone receptors in the vomeronasal neuron of the Swiss mouse" |
| Keywords: | "Amino Acid Sequence Animals Anthracenes/chemistry/metabolism Chromatography, Reverse-Phase/*methods Electrophoresis, Polyacrylamide Gel Mass Spectrometry Mice Molecular Sequence Data Receptors, Pheromone/*chemistry/isolation & purification/metabolism Spec;" |
| Notes: | "MedlineKannan, Soundarapandian Mareeswaran, Pandi Krishnan, Muthukalingan Achiraman, Shanmugam Spencer, Neil Nytingel, Balakrishnan F eng Research Support, Non-U.S. Gov't England 2010/12/31 Rapid Commun Mass Spectrom. 2011 Jan 30; 25(2):262-70. doi: 10.1002/rcm.4864" |
