| Title: | Validated single urinary assay designed for exposomic multi-class biomarkers of common environmental exposures |
| Author(s): | Jagani R; Pulivarthi D; Patel D; Wright RJ; Wright RO; Arora M; Wolff MS; Andra SS; |
| Address: | "Department of Environmental Medicine and Public Health, Icahn School of Medicine at Mount Sinai, New York, NY, USA. Institute for Exposomic Research, Senator Frank R. Lautenberg Environmental Health Sciences Laboratory, Icahn School of Medicine at Mount Sinai, Atran Lab, Floor 3, Room 002, 1428 Madison Avenue, New York, NY, 10029, USA. Texas Tech University Health Sciences Center, Amarillo, TX, USA. Kravis Children's Hospital, Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, NY, USA. Department of Environmental Medicine and Public Health, Icahn School of Medicine at Mount Sinai, New York, NY, USA. syam.andra@mssm.edu. Institute for Exposomic Research, Senator Frank R. Lautenberg Environmental Health Sciences Laboratory, Icahn School of Medicine at Mount Sinai, Atran Lab, Floor 3, Room 002, 1428 Madison Avenue, New York, NY, 10029, USA. syam.andra@mssm.edu" |
| DOI: | 10.1007/s00216-022-04159-4 |
| ISSN/ISBN: | 1618-2650 (Electronic) 1618-2642 (Print) 1618-2642 (Linking) |
| Abstract: | "Epidemiological studies often call for analytical methods that use a small biospecimen volume to quantify trace level exposures to environmental chemical mixtures. Currently, as many as 150 polar metabolites of environmental chemicals have been found in urine. Therefore, we developed a multi-class method for quantitation of biomarkers in urine. A single sample preparation followed by three LC injections was optimized in a proof-of-approach for a multi-class method. The assay was validated to quantify 50 biomarkers of exposure in urine, belonging to 7 chemical classes and 16 sub-classes. The classes represent metabolites of 12 personal care and consumer product chemicals (PCPs), 5 polycyclic aromatic hydrocarbons (PAHs), 5 organophosphate flame retardants (OPFRs), 18 pesticides, 5 volatile organic compounds (VOCs), 4 tobacco alkaloids, and 1 drug of abuse. Human urine (0.2 mL) was spiked with isotope-labeled internal standards, enzymatically deconjugated, extracted by solid-phase extraction, and analyzed using high-performance liquid chromatography-tandem mass spectrometry. The methanol eluate from the cleanup was split in half and the first half analyzed for PCPs, PAH, and OPFR on a Betasil C18 column; and pesticides and VOC on a Hypersil Gold AQ column. The second half was analyzed for tobacco smoke metabolites and a drug of abuse on a Synergi Polar RP column. Limits of detection ranged from 0.01 to 1.0 ng/mL of urine, with the majority |
| Keywords: | Biomarkers/urine Environmental Exposure/analysis *Flame Retardants/analysis Humans *Pesticides/analysis Tandem Mass Spectrometry/methods Biomarkers Biomonitoring Chemical mixtures Exposome Exposure assessment Multi-class assay; |
| Notes: | "MedlineJagani, Ravikumar Pulivarthi, Divya Patel, Dhavalkumar Wright, Rosalind J Wright, Robert O Arora, Manish Wolff, Mary S Andra, Syam S eng P30 ES023515/ES/NIEHS NIH HHS/ R01 ES026033/ES/NIEHS NIH HHS/ U2C ES026561/ES/NIEHS NIH HHS/ Germany 2022/06/27 Anal Bioanal Chem. 2022 Aug; 414(19):5943-5966. doi: 10.1007/s00216-022-04159-4. Epub 2022 Jun 27" |
