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PLoS One

Title:		Cth2 Protein Mediates Early Adaptation of Yeast Cells to Oxidative Stress Conditions
Author(s):		Castells-Roca L; Pijuan J; Ferrezuelo F; Belli G; Herrero E;
Address:		"Departament de Ciencies Mediques Basiques, Universitat de Lleida, IRBLleida,Lleida, Spain"
Journal Title:		PLoS One
Year:		2016
Volume:		20160129
Issue:		1
Page Number:		e0148204 -
DOI:		10.1371/journal.pone.0148204
ISSN/ISBN:		1932-6203 (Electronic) 1932-6203 (Linking)
Abstract:		"Cth2 is an mRNA-binding protein that participates in remodeling yeast cell metabolism in iron starvation conditions by promoting decay of the targeted molecules, in order to avoid excess iron consumption. This study shows that in the absence of Cth2 immediate upregulation of expression of several of the iron regulon genes (involved in high affinity iron uptake and intracellular iron redistribution) upon oxidative stress by hydroperoxide is more intense than in wild type conditions where Cth2 is present. The oxidative stress provokes a temporary increase in the levels of Cth2 (itself a member of the iron regulon). In such conditions Cth2 molecules accumulate at P bodies-like structures when the constitutive mRNA decay machinery is compromised. In addition, a null Deltacth2 mutant shows defects, in comparison to CTH2 wild type cells, in exit from alpha factor-induced arrest at the G1 stage of the cell cycle when hydroperoxide treatment is applied. The cell cycle defects are rescued in conditions that compromise uptake of external iron into the cytosol. The observations support a role of Cth2 in modulating expression of diverse iron regulon genes, excluding those specifically involved in the reductive branch of the high-affinity transport. This would result in immediate adaptation of the yeast cells to an oxidative stress, by controlling uptake of oxidant-promoting iron cations"
Keywords:		"Adaptation, Physiological/*genetics G1 Phase Cell Cycle Checkpoints/drug effects/genetics Gene Expression Profiling *Gene Expression Regulation, Fungal Hydrogen Peroxide/pharmacology Ion Transport/drug effects Iron/*metabolism Mating Factor Oxidation-Redu;"
Notes:		"MedlineCastells-Roca, Laia Pijuan, Jordi Ferrezuelo, Francisco Belli, Gemma Herrero, Enrique eng Research Support, Non-U.S. Gov't 2016/01/30 PLoS One. 2016 Jan 29; 11(1):e0148204. doi: 10.1371/journal.pone.0148204. eCollection 2016"